The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

The progress of drug discovery and development is paced by milestones reached in organic
synthesis. In the last decade, the advent of late-stage functionalization (LSF) reactions has …

Chemical protein modification has emerged as an invaluable tool for the development of
modified proteins. The complementary use of both genetic and chemical methods has …

Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …

Over the past 16 years, genetic code expansion and reprogramming in living organisms has
been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA …

Super‐resolution microscopy (SRM) greatly benefits from the ability to install small
photostable fluorescent labels into proteins. Genetic code expansion (GCE) technology …

Labeling of a protein with a specific dye or tag at defined positions is a critical step in tracing
the subtle behavior of the protein and assessing its cellular function. Over the last decade …

Bioorthogonal reactions have been widely used over the last 10 years for imaging,
detection, diagnostics, drug delivery, and biomaterials. Tetrazine reactions are a recently …

Engineering new functionality into living eukaryotic systems by enzyme evolution or de novo
protein design is a formidable challenge. Cells do not rely exclusively on DNA-based …

The wavelength-dependent conversion of two rapid photoinduced ligation reactions, ie, the
light activation of o-methylbenzaldehydes, leading to the formation of reactive o …