Plant-based biopharmaceutical engineering

L Eidenberger, B Kogelmann… - Nature Reviews …, 2023 - nature.com
Plants can be engineered to recombinantly produce high-quality proteins such as
therapeutic proteins and vaccines, also known as molecular farming. Molecular farming can …

[HTML][HTML] CRISPR/Cas genome editing in tomato improvement: Advances and applications

JK Tiwari, AK Singh, TK Behera - Frontiers in Plant Science, 2023 - frontiersin.org
The narrow genetic base of tomato poses serious challenges in breeding. Hence, with the
advent of clustered regularly interspaced short palindromic repeat (CRISPR)-associated …

Synthetic developmental biology: molecular tools to re-design plant shoots and roots

EG Kocaoglan, D Radhakrishnan… - Journal of …, 2023 - academic.oup.com
Plant morphology and anatomy strongly influence agricultural yield. Crop domestication has
strived for desirable growth and developmental traits, such as larger and more fruits and …

Challenges and advances towards the rational design of microalgal synthetic promoters in Chlamydomonas reinhardtii

A Milito, M Aschern, JL McQuillan… - Journal of Experimental …, 2023 - academic.oup.com
Microalgae hold enormous potential to provide a safe and sustainable source of high-value
compounds, acting as carbon-fixing biofactories that could help to mitigate rapidly …

[HTML][HTML] Knockout of phytoene desaturase gene using CRISPR/Cas9 in highbush blueberry

G Vaia, V Pavese, A Moglia, V Cristofori… - Frontiers in Plant …, 2022 - frontiersin.org
Among the New Plant Breeding Techniques (NPBTs), the CRISPR/Cas9 system represents
a useful tool for target gene editing, improving the traits of the plants rapidly. This technology …

RNA virus-mediated gene editing for tomato trait breeding

M Uranga, V Aragonés, A García, S Mirabel… - Horticulture …, 2024 - academic.oup.com
HIGHLIGHTS Generation of a transgenic Cas9-expressing line of tomato cv. Micro-Tom Use
of PVX for sgRNA delivery into Cas9-expressing Micro-Tom plants Heritable proof-of …

General guidelines for CRISPR/Cas-based genome editing in plants

E Aksoy, K Yildirim, M Kavas, C Kayihan… - Molecular Biology …, 2022 - Springer
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-
associated) technology is a versatile genome editing tool that has been used to improve …

Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects

D May, K Paldi, F Altpeter - The Plant Genome, 2023 - Wiley Online Library
Many of the world's most important crops are polyploid. The presence of more than two sets
of chromosomes within their nuclei and frequently aberrant reproductive biology in …

CRISPRi-based circuits to control gene expression in plants

MA Khan, G Herring, JY Zhu, M Oliva, E Fourie… - Nature …, 2024 - nature.com
The construction of synthetic gene circuits in plants has been limited by a lack of orthogonal
and modular parts. Here, we implement a CRISPR (clustered regularly interspaced short …

[HTML][HTML] Comparative analysis of the Squamosa Promoter Binding-Like (SPL) gene family in Nicotiana benthamiana and Nicotiana tabacum

C De Paola, V Garcia-Carpintero, M Vazquez-Vilar… - Plant Science, 2023 - Elsevier
Abstract SQUAMOSA PROMOTER BINDING-LIKE (SPL) proteins constitute a large family of
transcription factors known to play key roles in growth and developmental processes …