Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications

M Tan, C Liao, L Liang, X Yi, Z Zhou… - Frontiers in cellular and …, 2022 - frontiersin.org
After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people's lives. In
addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid …

Malaria diagnostic update: From conventional to advanced method

LE Fitri, T Widaningrum, AT Endharti… - Journal of Clinical …, 2022 - Wiley Online Library
Background Update diagnostic methods play essential roles in dealing with the current
global malaria situation and decreasing malaria incidence. Aim Global malaria control …

Various techniques for molecular and rapid detection of infectious and epidemic diseases

MH Shahrajabian, W Sun - Letters in Organic Chemistry, 2023 - benthamdirect.com
Polymerase chain reaction is an approach to make numerous copies of specific DNA. PCR
has been applied for the investigation of infectious sicknesses caused by viral, protozoan …

[HTML][HTML] Fully integrated sample-in-answer-out platform for viral detection using digital reverse transcription recombinase polymerase amplification (dRT-RPA)

I Seder, R Coronel-Tellez, SH Helalat, Y Sun - Biosensors and …, 2023 - Elsevier
Recombinase polymerase amplification (RPA) is one of the most promising diagnostic
methods for pathogen detection, owing to the simplified isothermal amplification technique …

Microfluidics for COVID-19: from current work to future perspective

Q Li, X Zhou, Q Wang, W Liu, C Chen - Biosensors, 2023 - mdpi.com
Spread of coronavirus disease 2019 (COVID-19) has significantly impacted the public health
and economic sectors. It is urgently necessary to develop rapid, convenient, and cost …

The laboratory diagnosis of malaria: a focus on the diagnostic assays in non-endemic areas

A Calderaro, G Piccolo, C Chezzi - International Journal of Molecular …, 2024 - mdpi.com
Even if malaria is rare in Europe, it is a medical emergency and programs for its control
should ensure both an early diagnosis and a prompt treatment within 24–48 h from the onset …

Combined recombinase polymerase amplification/rkDNA–graphene oxide probing system for detection of SARS-CoV-2

MH Choi, J Lee, YJ Seo - Analytica Chimica Acta, 2021 - Elsevier
The development of rapid, highly sensitive, and selective methods for the diagnosis of
infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) should help to …

Recombinase polymerase amplification for rapid detection of zoonotic pathogens: an overview

R Lv, N Lu, J Wang, Y Li, Y Qi - Zoonoses, 2022 - scienceopen.com
With the advent of molecular technology, several isothermal techniques for rapid detection of
zoonotic pathogens have been developed. Among them, recombinase polymerase …

Point-of-care test of blood Plasmodium RNA within a Pasteur pipette using a novel isothermal amplification without nucleic acid purification

L Xie, J Xu, L Fan, X Sun, Z Zheng - Infectious Diseases of Poverty, 2024 - mednexus.org
Background Resource-limited regions face a greater burden of infectious diseases due to
limited access to molecular tests, complicating timely diagnosis and management. Current …

Rapid detection of Mycobacterium tuberculosis based on antigen 85B via real‐time recombinase polymerase amplification

Y Xu, P Wu, H Zhang, J Li - Letters in applied microbiology, 2021 - academic.oup.com
Tuberculosis (TB), as a common infectious disease, still remains a severe challenge to
public health. Due to the unsatisfied clinical needs of currently available diagnostic vehicles …