Real-time PCR detection chemistry

E Navarro, G Serrano-Heras, MJ Castaño, J Solera - Clinica chimica acta, 2015 - Elsevier
Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …

Triplex DNA nanostructures: from basic properties to applications

Y Hu, A Cecconello, A Idili, F Ricci… - Angewandte Chemie …, 2017 - Wiley Online Library
Triplex nucleic acids have recently attracted interest as part of the rich “toolbox” of structures
used to develop DNA‐based nanostructures and materials. This Review addresses the use …

Self-protected DNAzyme walker with a circular bulging DNA shield for amplified imaging of miRNAs in living cells and mice

Y Gao, S Zhang, C Wu, Q Li, Z Shen, Y Lu, ZS Wu - ACS nano, 2021 - ACS Publications
Abnormal expression of miRNAs is often detected in various human cancers. DNAzyme
machines combined with gold nanoparticles (AuNPs) hold promise for detecting specific …

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

SA Bustin - Journal of molecular endocrinology, 2000 - jme.bioscientifica.com
To investigate whether the tumor suppressor gene PTEN affects the activity of the androgen
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …

Real-time PCR for mRNA quantitation

ML Wong, JF Medrano - Biotechniques, 2005 - Taylor & Francis
Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …

The real-time polymerase chain reaction

M Kubista, JM Andrade, M Bengtsson… - Molecular aspects of …, 2006 - Elsevier
The scientific, medical, and diagnostic communities have been presented the most powerful
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …

Sample dimensions influence strength and crystal plasticity

MD Uchic, DM Dimiduk, JN Florando, WD Nix - Science, 2004 - science.org
When a crystal deforms plastically, phenomena such as dislocation storage, multiplication,
motion, pinning, and nucleation occur over the submicron-to-nanometer scale. Here we …

The thermodynamics of DNA structural motifs

J SantaLucia Jr, D Hicks - Annu. Rev. Biophys. Biomol. Struct., 2004 - annualreviews.org
▪ Abstract DNA secondary structure plays an important role in biology, genotyping
diagnostics, a variety of molecular biology techniques, in vitro–selected DNA catalysts …

An overview of real-time quantitative PCR: applications to quantify cytokine gene expression

A Giulietti, L Overbergh, D Valckx, B Decallonne… - Methods, 2001 - Elsevier
The analysis of cytokine profiles helps to clarify functional properties of immune cells, both
for research and for clinical diagnosis. The real-time reverse transcription polymerase chain …

DNA biosensors and microarrays

A Sassolas, BD Leca-Bouvier, LJ Blum - Chemical reviews, 2008 - ACS Publications
Development of DNA biosensors and DNA microarrays has increased tremendously over
the past few years as demonstrated by the large number of scientific publications in this area …