Real-time PCR detection chemistry
E Navarro, G Serrano-Heras, MJ Castaño, J Solera - Clinica chimica acta, 2015 - Elsevier
Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …
applications. This technology merges the polymerase chain reaction chemistry with the use …
Triplex DNA nanostructures: from basic properties to applications
Triplex nucleic acids have recently attracted interest as part of the rich “toolbox” of structures
used to develop DNA‐based nanostructures and materials. This Review addresses the use …
used to develop DNA‐based nanostructures and materials. This Review addresses the use …
Self-protected DNAzyme walker with a circular bulging DNA shield for amplified imaging of miRNAs in living cells and mice
Abnormal expression of miRNAs is often detected in various human cancers. DNAzyme
machines combined with gold nanoparticles (AuNPs) hold promise for detecting specific …
machines combined with gold nanoparticles (AuNPs) hold promise for detecting specific …
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
SA Bustin - Journal of molecular endocrinology, 2000 - jme.bioscientifica.com
To investigate whether the tumor suppressor gene PTEN affects the activity of the androgen
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …
Real-time PCR for mRNA quantitation
ML Wong, JF Medrano - Biotechniques, 2005 - Taylor & Francis
Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …
The real-time polymerase chain reaction
M Kubista, JM Andrade, M Bengtsson… - Molecular aspects of …, 2006 - Elsevier
The scientific, medical, and diagnostic communities have been presented the most powerful
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …
Sample dimensions influence strength and crystal plasticity
When a crystal deforms plastically, phenomena such as dislocation storage, multiplication,
motion, pinning, and nucleation occur over the submicron-to-nanometer scale. Here we …
motion, pinning, and nucleation occur over the submicron-to-nanometer scale. Here we …
The thermodynamics of DNA structural motifs
J SantaLucia Jr, D Hicks - Annu. Rev. Biophys. Biomol. Struct., 2004 - annualreviews.org
▪ Abstract DNA secondary structure plays an important role in biology, genotyping
diagnostics, a variety of molecular biology techniques, in vitro–selected DNA catalysts …
diagnostics, a variety of molecular biology techniques, in vitro–selected DNA catalysts …
An overview of real-time quantitative PCR: applications to quantify cytokine gene expression
A Giulietti, L Overbergh, D Valckx, B Decallonne… - Methods, 2001 - Elsevier
The analysis of cytokine profiles helps to clarify functional properties of immune cells, both
for research and for clinical diagnosis. The real-time reverse transcription polymerase chain …
for research and for clinical diagnosis. The real-time reverse transcription polymerase chain …
DNA biosensors and microarrays
A Sassolas, BD Leca-Bouvier, LJ Blum - Chemical reviews, 2008 - ACS Publications
Development of DNA biosensors and DNA microarrays has increased tremendously over
the past few years as demonstrated by the large number of scientific publications in this area …
the past few years as demonstrated by the large number of scientific publications in this area …