The distinct roles of zinc finger CCHC-type (ZCCHC) superfamily proteins in the regulation of RNA metabolism

Y Wang, Y Yu, Y Pang, H Yu, W Zhang, X Zhao… - RNA biology, 2021 - Taylor & Francis
The zinc finger CCHC-type (ZCCHC) superfamily proteins, characterized with the consensus
sequence C-X2-C-X4-H-X4-C, are accepted to have high-affinity binding to single-stranded …

A structure-free method for quantifying conformational flexibility in proteins

VM Burger, DJ Arenas, CM Stultz - Scientific reports, 2016 - nature.com
All proteins sample a range of conformations at physiologic temperatures and this inherent
flexibility enables them to carry out their prescribed functions. A comprehensive …

The mysterious RAMP proteins and their roles in small RNA‐based immunity

R Wang, H Li - Protein Science, 2012 - Wiley Online Library
A new class of prokaryotic RNA binding proteins called Repeat Associated Mysterious
Proteins (RAMPs), has recently been identified. These proteins play key roles in a novel type …

Structure, phosphorylation and U2AF65 binding of the N-terminal domain of splicing factor 1 during 3′-splice site recognition

Y Zhang, T Madl, I Bagdiul, T Kern… - Nucleic acids …, 2013 - academic.oup.com
Recognition of the 3′-splice site is a key step in pre-mRNA splicing and accomplished by a
dynamic complex comprising splicing factor 1 (SF1) and the U2 snRNP auxiliary factor 65 …

U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs

JL Jenkins, AA Agrawal, A Gupta… - Nucleic acids …, 2013 - academic.oup.com
Degenerate splice site sequences mark the intron boundaries of pre-mRNA transcripts in
multicellular eukaryotes. The essential pre-mRNA splicing factor U2AF65 is faced with the …

Structure of phosphorylated SF1 bound to U2AF65 in an essential splicing factor complex

W Wang, A Maucuer, A Gupta, V Manceau… - Structure, 2013 - cell.com
The essential splicing factors U2AF 65 and SF1 cooperatively bind consensus sequences at
the 3′ end of introns. Phosphorylation of SF1 on a highly conserved" SPSP" motif …

Three RNA recognition motifs participate in RNA recognition and structural organization by the pro-apoptotic factor TIA-1

WJ Bauer, J Heath, JL Jenkins, CL Kielkopf - Journal of molecular biology, 2012 - Elsevier
T-cell intracellular antigen-1 (TIA-1) regulates developmental and stress-responsive
pathways through distinct activities at the levels of alternative pre-mRNA splicing and mRNA …

Contribution of IKBKE and IFIH1 gene variants to SLE susceptibility

C Wang, A Ahlford, N Laxman, G Nordmark… - Genes & …, 2013 - nature.com
The type I interferon system genes IKBKE and IFIH1 are associated with the risk of systemic
lupus erythematosus (SLE). To identify the sequence variants that are able to account for the …

Branch point identification and sequence requirements for intron splicing in Plasmodium falciparum

X Zhang, CA Tolzmann, M Melcher, BJ Haas… - Eukaryotic …, 2011 - Am Soc Microbiol
Splicing of mRNA is an ancient and evolutionarily conserved process in eukaryotic
organisms, but intron-exon structures vary. Plasmodium falciparum has an extreme AT …

SF1 phosphorylation enhances specific binding to U2AF65 and reduces binding to 3′-splice-site RNA

R Chatrikhi, W Wang, A Gupta, S Loerch, A Maucuer… - Biophysical …, 2016 - cell.com
Abstract Splicing factor 1 (SF1) recognizes 3′ splice sites of the major class of introns as a
ternary complex with U2AF 65 and U2AF 35 splicing factors. A conserved SPSP motif in a …