Sequencing DNA with nanopores: Troubles and biases

C Delahaye, J Nicolas - PloS one, 2021 - journals.plos.org
Oxford Nanopore Technologies'(ONT) long read sequencers offer access to longer DNA
fragments than previous sequencer generations, at the cost of a higher error rate. While …

Epitranscriptomics in parasitic protists: role of RNA chemical modifications in posttranscriptional gene regulation

C Catacalos, A Krohannon, S Somalraju… - PLoS …, 2022 - journals.plos.org
“Epitranscriptomics” is the new RNA code that represents an ensemble of posttranscriptional
RNA chemical modifications, which can precisely coordinate gene expression and …

Straglr: discovering and genotyping tandem repeat expansions using whole genome long-read sequences

R Chiu, IS Rajan-Babu, JM Friedman, I Birol - Genome Biology, 2021 - Springer
Tandem repeat (TR) expansion is the underlying cause of over 40 neurological disorders.
Long-read sequencing offers an exciting avenue over conventional technologies for …

A mixed community of skin microbiome representatives influences cutaneous processes more than individual members

KH Loomis, SK Wu, A Ernlund, K Zudock, A Reno… - Microbiome, 2021 - Springer
Background Skin, the largest organ of the human body by weight, hosts a diversity of
microorganisms that can influence health. The microbial residents of the skin are now …

MTG-Link: leveraging barcode information from linked-reads to assemble specific loci

A Guichard, F Legeai, D Tagu, C Lemaitre - BMC bioinformatics, 2023 - Springer
Background Local assembly with short and long reads has proven to be very useful in many
applications: reconstruction of the sequence of a locus of interest, gap-filling in draft …

Emergence of carbapenem resistance in persistent Shewanella algae bacteremia: the role of pdsS G547W mutation in adaptive subpopulation dynamics

YT Huang, PY Liu - Annals of Clinical Microbiology and Antimicrobials, 2024 - Springer
This study elucidates the in vivo genetic mechanisms contributing to the emerging
resistance to carbapenem in Shewanella algae through a lens of adaptive microbial …

Evaluating coverage bias in next-generation sequencing of Escherichia coli

S Gunasekera, S Abraham, M Stegger, S Pang… - Plos one, 2021 - journals.plos.org
Whole-genome sequencing is essential to many facets of infectious disease research.
However, technical limitations such as bias in coverage and tagmentation, and difficulties …