Fluorescence lifetime measurements and biological imaging
MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …
Intracellular coenzymes as natural biomarkers for metabolic activities and mitochondrial anomalies
AA Heikal - Biomarkers in medicine, 2010 - Taylor & Francis
Mitochondria play a pivotal role in energy metabolism, programmed cell death and oxidative
stress. Mutated mitochondrial DNA in diseased cells compromises the structure of key …
stress. Mutated mitochondrial DNA in diseased cells compromises the structure of key …
[HTML][HTML] Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the single-cell …
Reduced nicotinamide adenine dinucleotide, NADH, is a major electron donor in the
oxidative phosphorylation and glycolytic pathways in cells. As a result, there has been …
oxidative phosphorylation and glycolytic pathways in cells. As a result, there has been …
Opportunities for persistent luminescent nanoparticles in luminescence imaging of biological systems and photodynamic therapy
The use of luminescence in biological systems allows us to diagnose diseases and
understand cellular processes. Persistent luminescent materials have emerged as an …
understand cellular processes. Persistent luminescent materials have emerged as an …
[HTML][HTML] Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative
FS Ariola, Z Li, C Cornejo, R Bittman, AA Heikal - Biophysical journal, 2009 - cell.com
Abstract Cholesterol-rich, liquid-ordered (L o) domains are believed to be biologically
relevant, and yet detailed knowledge about them, especially in live cells under physiological …
relevant, and yet detailed knowledge about them, especially in live cells under physiological …
Analysis of the metabolic deterioration of ex vivo skin from ischemic necrosis through the imaging of intracellular NAD(P)H by multiphoton tomography and …
WY Sanchez, TW Prow, WH Sanchez… - … of biomedical optics, 2010 - spiedigitallibrary.org
Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies,
transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to …
transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to …
Photoinduced charge transfer in short-distance ferrocenylsubphthalocyanine dyads
PV Solntsev, KL Spurgin, JR Sabin, AA Heikal… - Inorganic …, 2012 - ACS Publications
Two new ferrocenylsubphthalocyanine dyads with ferrocenylmethoxide (2) and
ferrocenecarboxylate (3) substituents directly attached to the subphthalocyanine ligand via …
ferrocenecarboxylate (3) substituents directly attached to the subphthalocyanine ligand via …
Fluorescence dynamics of a FRET probe designed for crowding studies
Living cells are crowded with macromolecules and organelles. As a result, there is an urgent
need for molecular sensors for quantitative, site-specific assessment of the macromolecular …
need for molecular sensors for quantitative, site-specific assessment of the macromolecular …
FRET Analysis of ionic strength sensors in the hofmeister series of salt solutions using fluorescence lifetime measurements
Living cells are complex, crowded, and dynamic and continually respond to environmental
and intracellular stimuli. They also have heterogeneous ionic strength with …
and intracellular stimuli. They also have heterogeneous ionic strength with …
Crowding effects on energy-transfer efficiencies of hetero-FRET probes as measured using time-resolved fluorescence anisotropy
HJ Leopold, R Leighton, J Schwarz… - The Journal of …, 2018 - ACS Publications
Macromolecular crowding is prevalent in all living cells due to the presence of large
biomolecules and organelles. Cellular crowding is heterogeneous and is known to influence …
biomolecules and organelles. Cellular crowding is heterogeneous and is known to influence …