Fluorescence lifetime measurements and biological imaging

MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Intracellular coenzymes as natural biomarkers for metabolic activities and mitochondrial anomalies

AA Heikal - Biomarkers in medicine, 2010 - Taylor & Francis
Mitochondria play a pivotal role in energy metabolism, programmed cell death and oxidative
stress. Mutated mitochondrial DNA in diseased cells compromises the structure of key …

[HTML][HTML] Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the single-cell …

Q Yu, AA Heikal - Journal of Photochemistry and Photobiology B: Biology, 2009 - Elsevier
Reduced nicotinamide adenine dinucleotide, NADH, is a major electron donor in the
oxidative phosphorylation and glycolytic pathways in cells. As a result, there has been …

Opportunities for persistent luminescent nanoparticles in luminescence imaging of biological systems and photodynamic therapy

DL Fritzen, L Giordano, LCV Rodrigues… - Nanomaterials, 2020 - mdpi.com
The use of luminescence in biological systems allows us to diagnose diseases and
understand cellular processes. Persistent luminescent materials have emerged as an …

[HTML][HTML] Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative

FS Ariola, Z Li, C Cornejo, R Bittman, AA Heikal - Biophysical journal, 2009 - cell.com
Abstract Cholesterol-rich, liquid-ordered (L o) domains are believed to be biologically
relevant, and yet detailed knowledge about them, especially in live cells under physiological …

Analysis of the metabolic deterioration of ex vivo skin from ischemic necrosis through the imaging of intracellular NAD(P)H by multiphoton tomography and …

WY Sanchez, TW Prow, WH Sanchez… - … of biomedical optics, 2010 - spiedigitallibrary.org
Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies,
transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to …

Photoinduced charge transfer in short-distance ferrocenylsubphthalocyanine dyads

PV Solntsev, KL Spurgin, JR Sabin, AA Heikal… - Inorganic …, 2012 - ACS Publications
Two new ferrocenylsubphthalocyanine dyads with ferrocenylmethoxide (2) and
ferrocenecarboxylate (3) substituents directly attached to the subphthalocyanine ligand via …

Fluorescence dynamics of a FRET probe designed for crowding studies

M Currie, H Leopold, J Schwarz… - The Journal of …, 2017 - ACS Publications
Living cells are crowded with macromolecules and organelles. As a result, there is an urgent
need for molecular sensors for quantitative, site-specific assessment of the macromolecular …

FRET Analysis of ionic strength sensors in the hofmeister series of salt solutions using fluorescence lifetime measurements

RC Miller, CP Aplin, TM Kay, R Leighton… - The Journal of …, 2020 - ACS Publications
Living cells are complex, crowded, and dynamic and continually respond to environmental
and intracellular stimuli. They also have heterogeneous ionic strength with …

Crowding effects on energy-transfer efficiencies of hetero-FRET probes as measured using time-resolved fluorescence anisotropy

HJ Leopold, R Leighton, J Schwarz… - The Journal of …, 2018 - ACS Publications
Macromolecular crowding is prevalent in all living cells due to the presence of large
biomolecules and organelles. Cellular crowding is heterogeneous and is known to influence …