[HTML][HTML] Recent advances in CHO cell line development for recombinant protein production
B Tihanyi, L Nyitray - Drug Discovery Today: Technologies, 2020 - Elsevier
Recombinant proteins used in biomedical research, diagnostics and different therapies are
mostly produced in Chinese hamster ovary cells in the pharmaceutical industry. These …
mostly produced in Chinese hamster ovary cells in the pharmaceutical industry. These …
The secretory pathway–the key for unlocking the potential of Chinese hamster ovary cell factories for manufacturing therapeutic proteins
M Torres, H Hussain, AJ Dickson - Critical Reviews in …, 2023 - Taylor & Francis
Mammalian cell factories (in particular the CHO cell system) have been crucial in the rise of
biopharmaceuticals. Mammalian cells have compartmentalized organelles where intricate …
biopharmaceuticals. Mammalian cells have compartmentalized organelles where intricate …
Reshaping cell line development and CMC strategy for fast responses to pandemic outbreak
Z Zhang, J Chen, J Wang, Q Gao, Z Ma… - Biotechnology …, 2021 - Wiley Online Library
The global pandemic outbreak COVID‐19 (SARS‐COV‐2), has prompted many
pharmaceutical companies to develop vaccines and therapeutic biologics for its prevention …
pharmaceutical companies to develop vaccines and therapeutic biologics for its prevention …
A platform for context-specific genetic engineering of recombinant protein production by CHO cells
JF Cartwright, CL Arnall, YD Patel, NOW Barber… - Journal of …, 2020 - Elsevier
An increasing number of engineered therapeutic recombinant proteins with unpredictable
manufacturability are currently filling industrial cell line development pipelines. These …
manufacturability are currently filling industrial cell line development pipelines. These …
Tailoring translational strength using Kozak sequence variants improves bispecific antibody assembly and reduces product‐related impurities in CHO cells
N Blanco, AJ Williams, D Tang, D Zhan… - Biotechnology and …, 2020 - Wiley Online Library
Optimal production of bispecific antibodies (bsAb) requires efficient and tailored co‐
expression and assembly of two distinct heavy and two distinct light chains. Here, we …
expression and assembly of two distinct heavy and two distinct light chains. Here, we …
Evaluation of two transposases for improving expression of recombinant proteins in Chinese hamster ovary cell stable pools by co‐transfection and supertransfection …
M Lenser, HG Ngo, L Sarrafha… - Biotechnology …, 2024 - Wiley Online Library
Transposons are genetic elements capable of cutting and pasting genes of interest via the
action of a transposase and offer many advantages over random or targeted integration of …
action of a transposase and offer many advantages over random or targeted integration of …
Repressing expression of difficult‐to‐express recombinant proteins during the selection process increases productivity of CHO stable pools
JS Maltais, S Lord‐Dufour, A Morasse… - Biotechnology and …, 2023 - Wiley Online Library
More than half of licensed therapeutic recombinant proteins (r‐proteins) are manufactured
using constitutively‐expressing, stably‐transfected Chinese hamster ovary (CHO) clones …
using constitutively‐expressing, stably‐transfected Chinese hamster ovary (CHO) clones …
[HTML][HTML] Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and …
JL Schloßhauer, L Tholen, A Körner, S Kubick… - Synthetic and Systems …, 2024 - Elsevier
Chinese hamster ovary (CHO) cells are crucial in biopharmaceutical production due to their
scalability and capacity for human-like post-translational modifications. However, toxic …
scalability and capacity for human-like post-translational modifications. However, toxic …
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cell expression system
AC Szkodny, KH Lee - Biotechnology Progress, 2024 - Wiley Online Library
Monoclonal antibodies (mAbs) are often engineered at the sequence level for improved
clinical performance yet are rarely evaluated prior to candidate selection for their …
clinical performance yet are rarely evaluated prior to candidate selection for their …
Chromatin Accessibility Plays an Important Epigenetic Role on Antibody Expression From CMV Promoter and DNA Elements Flanking the CHO TI Host Landing‐Pad
K Ganapathy, A McKay, S Durinck, M Shi… - Biotechnology …, 2024 - Wiley Online Library
Targeted integration (TI) Chinese hamster ovary (CHO) platforms are commonly used for
protein expression. However, the impact of epigenetic modifications on protein expression in …
protein expression. However, the impact of epigenetic modifications on protein expression in …