Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are produced in many
places in living cells and at an increased rate during biotic or abiotic stress. ROS and RNS …

Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been
successfully used to investigate a plethora of protein-related questions. The method, which …

“Footprinting” refers to assays that examine ligand binding and conformational changes by
determining the solvent accessibility of the backbone, bases, or side chain structures of …

Targeted cancer therapies, which act on specific cancer-associated molecular targets, are
predominantly inhibitors of oncogenic kinases. While these drugs have achieved some …

The Photosystem II reaction center is vulnerable to photoinhibition. The D1 and D2 proteins,
lying at the core of the photosystem, are susceptible to oxidative modification by reactive …

Structural proteomics approaches using mass spectrometry are increasingly used in biology
to examine the composition and structure of macromolecules. Hydroxyl radical–mediated …

Fast photochemical oxidation of proteins (FPOP) is a chemical footprinting method whereby
exposed amino-acid residues are covalently labeled by oxidation with hydroxyl radicals …

Using mass spectrometry (MS) to obtain information about a higher order structure of protein
requires that a protein's structural properties are encoded into the mass of that protein …

Mass spectrometry (MS) plays a central role in studies on protein structure and dynamics.
This review highlights some of the recent developments in this area, with focus on …