Germline development varies significantly across metazoans. However, mammalian
primordial germ cell (PGC) development has key conserved landmarks, including a critical …

The Weismann barrier has long been regarded as a basic tenet of biology. However, upon
close examination of its historical origins and August Weismann's own writings, questions …

Germ cells are essential to pass DNA from one generation to the next. In human
reproduction, germ cell development begins with the specification of primordial germ cells …

Human germ cells perpetuate human genetic and epigenetic information. However, the
underlying mechanism remains elusive, due to a lack of appropriate experimental systems …

Investigations of the human germline and programming are challenging because of limited
access to embryonic material. However, the pig as a model may provide insights into …

In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem
cell-derived PGC model, has proved challenging due to rapid loss of primordial germ cell …

Currently an in vitro model that fully recapitulates the human embryonic gonad is lacking.
Here we describe a fully defined feeder-free protocol to generate early testis-like cells with …

Mammalian female reproduction requires a functional ovary. Competence of the ovary is
determined by the quality of its basic unit–ovarian follicles. A normal follicle consists of an …

The germline provides the genetic and non-genetic information that passes from one
generation to the next. Given this important role in species propagation, egg and sperm …

In many non-mammalian organisms, a population of germ-line stem cells supports
continuing production of gametes during post-natal life, and germ-line stem cells are also …