Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Genome‐modification technologies enable the rational engineering and perturbation of
biological systems. Historically, these methods have been limited to gene insertions or …

Engineering cellular metabolism for improved production of valuable chemicals requires
extensive modulation of bacterial genome to explore complex genetic spaces. Here, we …

We describe the construction and characterization of a genomically recoded organism
(GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with …

Genetically modified organisms (GMOs) are increasingly deployed at large scales and in
open environments. Genetic biocontainment strategies are needed to prevent unintended …

Genetically modified organisms (GMOs) are increasingly used in research and industrial
systems to produce high-value pharmaceuticals, fuels and chemicals. Genetic isolation and …

Although the genetic code is redundant, synonymous codons for the same amino acid are
not used with equal frequencies in genomes, a phenomenon termed “codon usage bias.” …

Genome engineering methods in E. coli allow for easy to perform manipulations of the
chromosome in vivo with the assistance of the λ-Red recombinase system. These methods …

A bottleneck in metabolic engineering and systems biology approaches is the lack of
efficient genome engineering technologies. Here, we combine CRISPR/Cas9 and λ Red …

Recombination-mediated genetic engineering, also known as recombineering, is the
genomic incorporation of homologous single-stranded or double-stranded DNA into …