Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

H Li, Y Xie, F Chen, H Bai, L Xiu, X Zhou… - Chemical Society …, 2023 - pubs.rsc.org
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …

CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics

Z Weng, Z You, J Yang, N Mohammad… - Angewandte Chemie …, 2023 - Wiley Online Library
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …

A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a …

L Ma, W Zhang, L Yin, Y Li, J Zhuang, L Shen… - Journal of Hazardous …, 2023 - Elsevier
The pandemic of COVID-19 creates an imperative need for sensitive and portable detection
of SARS-CoV-2. We devised a SERS-read, CRISPR/Cas-powered nanobioassay, termed as …

Polyoxometalates: Cascading Functionality to Unique On‐Off Organic Photoelectrochemical Transistor Operation

FZ Chen, L Hou, Y Gao, JY Zhou… - Advanced Functional …, 2024 - Wiley Online Library
While existing polyoxometalates (POMs) are predominantly utilized in a range of energy
devices, their potential in bioelectronics and optoelectronics, particularly in organic …

CRISPR‐Mediated Profiling of Viral RNA at Single‐Nucleotide Resolution

D Chen, W Huang, Y Zhang, B Chen… - Angewandte Chemie …, 2023 - Wiley Online Library
Mass pathogen screening is critical to preventing the outbreaks and spread of infectious
diseases. The large‐scale epidemic of COVID‐19 and the rapid mutation of the severe acute …

CRISPR/Cas-powered amplification-free detection of nucleic acids: current state of the art, challenges, and futuristic perspectives

Y Li, Y Liu, X Tang, J Qiao, J Kou, S Man, L Zhu… - ACS …, 2023 - ACS Publications
CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …

A novel cascade signal amplification strategy integrating CRISPR/Cas13a and branched hybridization chain reaction for ultra-sensitive and specific SERS detection of …

J Zhang, C Song, Y Zhu, H Gan, X Fang, Q Peng… - Biosensors and …, 2023 - Elsevier
The molecular diagnosis of disease by high-sensitively and specifically detecting extremely
trace amounts of nucleic acid biomarkers in biological samples is still a great challenge, and …

CRISPR-Cas13a system: A novel tool for molecular diagnostics

L Zhao, M Qiu, X Li, J Yang, J Li - Frontiers in Microbiology, 2022 - frontiersin.org
The clustered regularly interspaced short palindromic repeats (CRISPR) system is a natural
adaptive immune system of prokaryotes. The CRISPR-Cas system is currently divided into …

CRISPR-based biosensors for human health: A novel strategy to detect emerging infectious diseases

Y He, Q Hu, S San, T Kasputis, MGD Splinter… - TrAC Trends in …, 2023 - Elsevier
Infectious diseases (such as sepsis, influenza, and malaria), caused by various pathogenic
bacteria and viruses, are widespread across the world. Early and rapid detection of disease …

PddCas: A polydisperse droplet digital CRISPR/Cas-based assay for the rapid and ultrasensitive amplification-free detection of viral DNA/RNA

Y Xue, X Luo, W Xu, K Wang, M Wu, L Chen… - Analytical …, 2022 - ACS Publications
Clustered regularly interspaced short palindromic repeats (CRISPR)-based assays have
been an emerging diagnostic technology for pathogen diagnosis. In this work, we developed …