Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a …
L Ma, W Zhang, L Yin, Y Li, J Zhuang, L Shen… - Journal of Hazardous …, 2023 - Elsevier
The pandemic of COVID-19 creates an imperative need for sensitive and portable detection
of SARS-CoV-2. We devised a SERS-read, CRISPR/Cas-powered nanobioassay, termed as …
of SARS-CoV-2. We devised a SERS-read, CRISPR/Cas-powered nanobioassay, termed as …
Polyoxometalates: Cascading Functionality to Unique On‐Off Organic Photoelectrochemical Transistor Operation
FZ Chen, L Hou, Y Gao, JY Zhou… - Advanced Functional …, 2024 - Wiley Online Library
While existing polyoxometalates (POMs) are predominantly utilized in a range of energy
devices, their potential in bioelectronics and optoelectronics, particularly in organic …
devices, their potential in bioelectronics and optoelectronics, particularly in organic …
CRISPR‐Mediated Profiling of Viral RNA at Single‐Nucleotide Resolution
D Chen, W Huang, Y Zhang, B Chen… - Angewandte Chemie …, 2023 - Wiley Online Library
Mass pathogen screening is critical to preventing the outbreaks and spread of infectious
diseases. The large‐scale epidemic of COVID‐19 and the rapid mutation of the severe acute …
diseases. The large‐scale epidemic of COVID‐19 and the rapid mutation of the severe acute …
CRISPR/Cas-powered amplification-free detection of nucleic acids: current state of the art, challenges, and futuristic perspectives
Y Li, Y Liu, X Tang, J Qiao, J Kou, S Man, L Zhu… - ACS …, 2023 - ACS Publications
CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …
repositioned in nucleic acid detection. A preamplification step is usually required to improve …
A novel cascade signal amplification strategy integrating CRISPR/Cas13a and branched hybridization chain reaction for ultra-sensitive and specific SERS detection of …
J Zhang, C Song, Y Zhu, H Gan, X Fang, Q Peng… - Biosensors and …, 2023 - Elsevier
The molecular diagnosis of disease by high-sensitively and specifically detecting extremely
trace amounts of nucleic acid biomarkers in biological samples is still a great challenge, and …
trace amounts of nucleic acid biomarkers in biological samples is still a great challenge, and …
CRISPR-Cas13a system: A novel tool for molecular diagnostics
L Zhao, M Qiu, X Li, J Yang, J Li - Frontiers in Microbiology, 2022 - frontiersin.org
The clustered regularly interspaced short palindromic repeats (CRISPR) system is a natural
adaptive immune system of prokaryotes. The CRISPR-Cas system is currently divided into …
adaptive immune system of prokaryotes. The CRISPR-Cas system is currently divided into …
CRISPR-based biosensors for human health: A novel strategy to detect emerging infectious diseases
Infectious diseases (such as sepsis, influenza, and malaria), caused by various pathogenic
bacteria and viruses, are widespread across the world. Early and rapid detection of disease …
bacteria and viruses, are widespread across the world. Early and rapid detection of disease …
PddCas: A polydisperse droplet digital CRISPR/Cas-based assay for the rapid and ultrasensitive amplification-free detection of viral DNA/RNA
Y Xue, X Luo, W Xu, K Wang, M Wu, L Chen… - Analytical …, 2022 - ACS Publications
Clustered regularly interspaced short palindromic repeats (CRISPR)-based assays have
been an emerging diagnostic technology for pathogen diagnosis. In this work, we developed …
been an emerging diagnostic technology for pathogen diagnosis. In this work, we developed …