Cross-linking mass spectrometry for investigating protein conformations and protein–protein interactions─ a method for all seasons
L Piersimoni, PL Kastritis, C Arlt, A Sinz - Chemical Reviews, 2021 - ACS Publications
Mass spectrometry (MS) has become one of the key technologies of structural biology. In this
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …
Mass spectrometry-based protein footprinting for higher-order structure analysis: fundamentals and applications
Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …
functions. Understanding the complexities of protein higher-order structures and dynamics …
Crosslinking and mass spectrometry: an integrated technology to understand the structure and function of molecular machines
A Leitner, M Faini, F Stengel, R Aebersold - Trends in biochemical …, 2016 - cell.com
In recent years, chemical crosslinking of protein complexes and the identification of
crosslinked residues by mass spectrometry (XL-MS; sometimes abbreviated as CX-MS) has …
crosslinked residues by mass spectrometry (XL-MS; sometimes abbreviated as CX-MS) has …
Cryo-EM structure of SWI/SNF complex bound to a nucleosome
The chromatin-remodelling complex SWI/SNF is highly conserved and has critical roles in
various cellular processes, including transcription and DNA-damage repair,. It hydrolyses …
various cellular processes, including transcription and DNA-damage repair,. It hydrolyses …
A cross-linking/mass spectrometry workflow based on MS-cleavable cross-linkers and the MeroX software for studying protein structures and protein–protein …
C Iacobucci, M Götze, CH Ihling, C Piotrowski, C Arlt… - Nature protocols, 2018 - nature.com
Chemical cross-linking in combination with mass spectrometric analysis of the created cross-
linked products is an emerging technology aimed at deriving valuable structural information …
linked products is an emerging technology aimed at deriving valuable structural information …
Cryo-EM structure of the fork protection complex bound to CMG at a replication fork
The eukaryotic replisome, organized around the Cdc45-MCM-GINS (CMG) helicase,
orchestrates chromosome replication. Multiple factors associate directly with CMG, including …
orchestrates chromosome replication. Multiple factors associate directly with CMG, including …
Recent progress in mass spectrometry-based strategies for elucidating protein–protein interactions
Protein–protein interactions are fundamental to various aspects of cell biology with many
protein complexes participating in numerous fundamental biological processes such as …
protein complexes participating in numerous fundamental biological processes such as …
Leveraging crosslinking mass spectrometry in structural and cell biology
A Graziadei, J Rappsilber - Structure, 2022 - cell.com
Crosslinking mass spectrometry (crosslinking-MS) is a versatile tool providing structural
insights into protein conformation and protein-protein interactions. Its medium-resolution …
insights into protein conformation and protein-protein interactions. Its medium-resolution …
Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes
A Leitner, LA Joachimiak… - Proceedings of the …, 2014 - National Acad Sciences
The study of proteins and protein complexes using chemical cross-linking followed by the
MS identification of the cross-linked peptides has found increasingly widespread use in …
MS identification of the cross-linked peptides has found increasingly widespread use in …
The interactome of intact mitochondria by cross-linking mass spectrometry provides evidence for coexisting respiratory supercomplexes
Mitochondria exert an immense amount of cytophysiological functions, but the structural
basis of most of these processes is still poorly understood. Here we use cross-linking mass …
basis of most of these processes is still poorly understood. Here we use cross-linking mass …