After over 30 years of advances, multiphoton microscopy (MPM) is now instrumental in a
wide range of in vivo biological imaging applications. However, it has, until recently …

In the past decade, the idea that single populations of neurons support cognition and
behavior has gradually given way to the realization that connectivity matters and that …

Two-photon, single-cell resolution optogenetics based on holographic light-targeting
approaches enables the generation of precise spatiotemporal neuronal activity patterns and …

For generations researchers have been observing the dynamic processes of life through the
lens of a microscope. This has offered tremendous insights into biological phenomena that …

Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional
imaging in zebrafish larvae. Conventional one-photon LSFM can however induce …

Fluorescence microscopy is essential for studying living cells, tissues and organisms.
However, the fluorescent light that switches on fluorescent molecules also harms the …

The inherent constraints on resolution, speed and field of view have hindered the
development of high-speed, three-dimensional microscopy techniques over large scales …

Tissues are heterogeneous with respect to cellular and non-cellular components and in the
dynamic interactions between these elements. To study the behaviour and fate of individual …

Nondestructive pathology based on three-dimensional (3D) optical microscopy holds
promise as a complement to traditional destructive hematoxylin and eosin (H&E) stained …

ABSTRACT A better understanding of cell–cell and cell–niche interactions is crucial to
comprehend the complexity of inflammatory or pathophysiological scenarios such as tissue …