Advances on size exclusion chromatography and applications on the analysis of protein biopharmaceuticals and protein aggregates: a mini review
G Brusotti, E Calleri, R Colombo, G Massolini… - Chromatographia, 2018 - Springer
Biopharmaceutical drugs are large, complex protein molecules derived from living cells. The
characterization of the protein-based products is a complicated and challenging task …
characterization of the protein-based products is a complicated and challenging task …
Fundamentals and applications of incorporating chromatographic separations with ion mobility-mass spectrometry
KA Morrison, BH Clowers - TrAC Trends in Analytical Chemistry, 2019 - Elsevier
Mass spectrometry (MS) is an extremely powerful analytical technique capable of yielding
chemical composition with high levels of speed, sensitivity, and selectivity. However …
chemical composition with high levels of speed, sensitivity, and selectivity. However …
Chaotropic effects in sub/supercritical fluid chromatography via ammonium hydroxide in water-rich modifiers: enabling separation of peptides and highly polar …
Chromatographic separation, analysis and characterization of complex highly polar analyte
mixtures can often be very challenging using conventional separation approaches. Analysis …
mixtures can often be very challenging using conventional separation approaches. Analysis …
Hydrogen–deuterium exchange mass spectrometry with integrated size-exclusion chromatography for analysis of complex protein samples
The growing use of hydrogen–deuterium exchange mass spectrometry (HDX-MS) for
studying membrane proteins, large protein assemblies, and highly disulfide-bonded species …
studying membrane proteins, large protein assemblies, and highly disulfide-bonded species …
Use of MALDI-MS combined with differential hydrogen–deuterium exchange for semiautomated protein global conformational screening
GF Pirrone, H Wang, N Canfield, AS Chin… - Analytical …, 2017 - ACS Publications
Matrix-assisted laser desorption/ionization (MALDI) coupled with a time-of-flight (TOF) mass-
spectrometry (MS) detector is acknowledged to be very useful for analysis of biological …
spectrometry (MS) detector is acknowledged to be very useful for analysis of biological …
Use of MALDI-MS with solid-state hydrogen deuterium exchange for semi-automated assessment of peptide and protein physical stability in lyophilized solids
SR Kabaria, I Mangion, AA Makarov, GF Pirrone - Analytica chimica acta, 2019 - Elsevier
Biological therapeutics are established as major contributors to the pharmaceutical pipeline.
Many of these biological drugs are lyophilized to preserve their conformation and reduce …
Many of these biological drugs are lyophilized to preserve their conformation and reduce …
Interrogation of solution conformation of complex macrocyclic peptides utilizing a combined SEC-HDX-MS, circular dichroism, and NMR workflow
Recent technological and synthetic advances have led to a resurgence in the exploration of
peptides as potential therapeutics. Understanding peptide conformation in both free and …
peptides as potential therapeutics. Understanding peptide conformation in both free and …
Strengthening of porcine plasma protein superabsorbent materials through a solubilization-freeze-drying process
The replacement of common acrylic derivatives by biodegradable materials in the
formulation of superabsorbent materials would lessen the associated environmental impact …
formulation of superabsorbent materials would lessen the associated environmental impact …
[PDF][PDF] Towards Probing Structure and Function Relationships of Proteins in Complex Sample Types Using HX-MS
J Sheff - 2017 - prism.ucalgary.ca
Towards Probing Structure and Function Relationships of Proteins in Complex Sample Types
Using HX-MS Page 1 University of Calgary PRISM Repository https://prism.ucalgary.ca The …
Using HX-MS Page 1 University of Calgary PRISM Repository https://prism.ucalgary.ca The …