Spatially resolved mass spectrometry at the single cell: recent innovations in proteomics and metabolomics
MJ Taylor, JK Lukowski… - Journal of the American …, 2021 - ACS Publications
Biological systems are composed of heterogeneous populations of cells that
intercommunicate to form a functional living tissue. Biological function varies greatly across …
intercommunicate to form a functional living tissue. Biological function varies greatly across …
3D cell culture models as recapitulators of the tumor microenvironment for the screening of anti-cancer drugs
Simple Summary Three-dimensional (3D) cell culture models have been proposed as
alternatives for initial drug screening to increase drug development efficiency. In addition to …
alternatives for initial drug screening to increase drug development efficiency. In addition to …
3D printing for chemical, pharmaceutical and biological applications
Abstract 3D printing is becoming increasingly prevalent in modern chemistry laboratories.
This technology provides chemists with the ability to design, prototype and print functional …
This technology provides chemists with the ability to design, prototype and print functional …
Promising applications of tumor spheroids and organoids for personalized medicine
Simple Summary Presently, the investigation of the tumor is carried out using different
models. One of the promising areas is the creation of 3D tumor models, such as spheroids …
models. One of the promising areas is the creation of 3D tumor models, such as spheroids …
Hybrid-DIA: intelligent data acquisition integrates targeted and discovery proteomics to analyze phospho-signaling in single spheroids
Achieving sufficient coverage of regulatory phosphorylation sites by mass spectrometry (MS)-
based phosphoproteomics for signaling pathway reconstitution is challenging, especially …
based phosphoproteomics for signaling pathway reconstitution is challenging, especially …
Three-dimensional in vitro cell culture models for efficient drug discovery: Progress so far and future prospects
Despite tremendous advancements in technologies and resources, drug discovery still
remains a tedious and expensive process. Though most cells are cultured using 2D …
remains a tedious and expensive process. Though most cells are cultured using 2D …
Comparison of in-solution, FASP, and S-trap based digestion methods for bottom-up proteomic studies
KR Ludwig, MM Schroll… - Journal of proteome …, 2018 - ACS Publications
Bottom-up proteomic strategies rely on efficient digestion of proteins into peptides for mass
spectrometry analysis. In-solution and filter-based strategies are commonly used for …
spectrometry analysis. In-solution and filter-based strategies are commonly used for …
Short-term 3D culture systems of various complexity for treatment optimization of colorectal carcinoma
M Zoetemelk, M Rausch, DJ Colin, O Dormond… - Scientific reports, 2019 - nature.com
Abstract Three-dimensional (3D) cultures have the potential to increase the predictive value
of pre-clinical drug research and bridge the gap towards anticipating clinical outcome of …
of pre-clinical drug research and bridge the gap towards anticipating clinical outcome of …
Review of 3D cell culture with analysis in microfluidic systems
A review with 105 references that analyzes the emerging research area of 3D cell culture in
microfluidic platforms with integrated detection schemes. Over the last several decades a …
microfluidic platforms with integrated detection schemes. Over the last several decades a …
Deep learning and 3D-DESI imaging reveal the hidden metabolic heterogeneity of cancer
Visual inspection of tumour tissues does not reveal the complex metabolic changes that
differentiate cancer and its sub-types from healthy tissues. Mass spectrometry imaging …
differentiate cancer and its sub-types from healthy tissues. Mass spectrometry imaging …