Current applications and future perspective of CRISPR/Cas9 gene editing in cancer

SW Wang, C Gao, YM Zheng, L Yi, JC Lu, XY Huang… - Molecular cancer, 2022 - Springer
Clustered regularly interspaced short palindromic repeats (CRISPR) system provides
adaptive immunity against plasmids and phages in prokaryotes. This system inspires the …

Application of the CRISPR/Cas9-based gene editing technique in basic research, diagnosis, and therapy of cancer

H Zhang, C Qin, C An, X Zheng, S Wen, W Chen, X Liu… - Molecular cancer, 2021 - Springer
Abstract The 2020 Nobel Prize in Chemistry was awarded to Emmanuelle Charpentier and
Jennifer Doudna for the development of the Clustered regularly interspaced short …

Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing

ML Leibowitz, S Papathanasiou, PA Doerfler… - Nature …, 2021 - nature.com
Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …

Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics

M Hu, Z Qiu, Z Bi, T Tian, Y Jiang… - Proceedings of the …, 2022 - National Acad Sciences
CRISPR diagnostics based on nucleic acid amplification faces barriers to its commercial
use, such as contamination risks and insufficient sensitivity. Here, we propose a robust …

Loop extrusion as a mechanism for formation of DNA damage repair foci

C Arnould, V Rocher, AL Finoux, T Clouaire, K Li… - Nature, 2021 - nature.com
The repair of DNA double-strand breaks (DSBs) is essential for safeguarding genome
integrity. When a DSB forms, the PI3K-related ATM kinase rapidly triggers the establishment …

DNA repair pathway choices in CRISPR-Cas9-mediated genome editing

C Xue, EC Greene - Trends in Genetics, 2021 - cell.com
Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …

The role of chromatin loop extrusion in antibody diversification

Y Zhang, X Zhang, HQ Dai, H Hu, FW Alt - Nature Reviews Immunology, 2022 - nature.com
Cohesin mediates chromatin loop formation across the genome by extruding chromatin
between convergently oriented CTCF-binding elements. Recent studies indicate that …

Light‐Start CRISPR‐Cas12a Reaction with Caged crRNA Enables Rapid and Sensitive Nucleic Acid Detection

M Hu, R Liu, Z Qiu, F Cao, T Tian, Y Lu… - Angewandte …, 2023 - Wiley Online Library
The clustered regularly interspaced short palindromic repeats (CRISPR) system is a
promising platform for nucleic acid detection. Regulating the CRISPR reaction would be …

HLTF disrupts Cas9-DNA post-cleavage complexes to allow DNA break processing

G Reginato, MR Dello Stritto, Y Wang, J Hao… - Nature …, 2024 - nature.com
The outcome of CRISPR-Cas-mediated genome modifications is dependent on DNA double-
strand break (DSB) processing and repair pathway choice. Homology-directed repair (HDR) …

Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance

R Schep, EK Brinkman, C Leemans, X Vergara… - Molecular cell, 2021 - cell.com
DNA double-strand break (DSB) repair is mediated by multiple pathways. It is thought that
the local chromatin context affects the pathway choice, but the underlying principles are …