[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Improvement of enzyme activity, stability and selectivity via immobilization techniques

C Mateo, JM Palomo, G Fernandez-Lorente… - Enzyme and microbial …, 2007 - Elsevier
In spite of their excellent catalytic properties, enzyme properties have to be usually improved
before their implementation at industrial scale (where many cycles of high yield processes …

Enzyme stability and stabilization—aqueous and non-aqueous environment

PV Iyer, L Ananthanarayan - Process biochemistry, 2008 - Elsevier
Enzyme stabilization has notable importance due to increasing number of enzyme
applications. Stabilization of enzymes in order to realize their full potential as catalysts is …

Exopolymeric substances of sulfate‐reducing bacteria: interactions with calcium at alkaline pH and implication for formation of carbonate minerals

O Braissant, AW Decho, C Dupraz, C Glunk… - …, 2007 - Wiley Online Library
Sulfate‐reducing bacteria (SRB) have been recognized as key players in the precipitation of
calcium carbonate in lithifying microbial communities. These bacteria increase the alkalinity …

Enzymes on material surfaces

JN Talbert, JM Goddard - Colloids and Surfaces B: Biointerfaces, 2012 - Elsevier
Enzyme interactions with material surfaces are of interest for industrial food and
pharmaceutical transformations, biosensors, artificial cells, cell free reactions, drug and …

Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins

C Mateo, JM Palomo, M Fuentes, L Betancor… - Enzyme and Microbial …, 2006 - Elsevier
Glyoxyl agarose is constituted by quite thick agarose fibres containing a large number of
very stable aldehyde groups attached to the support by very short spacer arms. Under …

Co-immobilization of multiple enzymes by metal coordinated nucleotide hydrogel nanofibers: improved stability and an enzyme cascade for glucose detection

H Liang, S Jiang, Q Yuan, G Li, F Wang, Z Zhang, J Liu - Nanoscale, 2016 - pubs.rsc.org
Preserving enzyme activity and promoting synergistic activity via co-localization of multiple
enzymes are key topics in bionanotechnology, materials science, and analytical chemistry …

Carrier free co-immobilization of alpha amylase, glucoamylase and pullulanase as combined cross-linked enzyme aggregates (combi-CLEAs): a tri-enzyme …

S Talekar, A Pandharbale, M Ladole, S Nadar… - Bioresource …, 2013 - Elsevier
A tri-enzyme biocatalyst “combi-CLEAs” with starch hydrolytic activity was prepared from
commercially available alpha amylase, glucoamylase and pullulanase preparations by …

Functionalized κ-carrageenan/hyperbranched poly (amidoamine) for protease immobilization: thermodynamics and stability studies

GEA Awad, AF Ghanem, WAA Wahab… - International journal of …, 2020 - Elsevier
Hyperbranched polyamidoamine (PAMAM) has a high density of surface amino groups. This
feature was exploited in the surface modification of κ-carrageenan gel beads for successful …

A bibliometric analysis and review of pullulan-degrading enzymes—past and current trends

UM Kahar, NA Latif, SI Amran, KJ Liew, KM Goh - Catalysts, 2022 - mdpi.com
Starch and pullulan degrading enzymes are essential industrial biocatalysts. Pullulan-
degrading enzymes are grouped into pullulanases (types I and type II) and pullulan …