Phase transitions of associative biomacromolecules

RV Pappu, SR Cohen, F Dar, M Farag, M Kar - Chemical Reviews, 2023 - ACS Publications
Multivalent proteins and nucleic acids, collectively referred to as multivalent associative
biomacromolecules, provide the driving forces for the formation and compositional …

The molecular basis for cellular function of intrinsically disordered protein regions

AS Holehouse, BB Kragelund - Nature Reviews Molecular Cell Biology, 2024 - nature.com
Intrinsically disordered protein regions exist in a collection of dynamic interconverting
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …

Modulating biomolecular condensates: a novel approach to drug discovery

DM Mitrea, M Mittasch, BF Gomes, IA Klein… - Nature Reviews Drug …, 2022 - nature.com
In the past decade, membraneless assemblies known as biomolecular condensates have
been reported to play key roles in many cellular functions by compartmentalizing specific …

Phase-separating RNA-binding proteins form heterogeneous distributions of clusters in subsaturated solutions

M Kar, F Dar, TJ Welsh, LT Vogel… - Proceedings of the …, 2022 - National Acad Sciences
Macromolecular phase separation is thought to be one of the processes that drives the
formation of membraneless biomolecular condensates in cells. The dynamics of phase …

Phase separation drives aberrant chromatin looping and cancer development

JH Ahn, ES Davis, TA Daugird, S Zhao, IY Quiroga… - Nature, 2021 - nature.com
The development of cancer is intimately associated with genetic abnormalities that target
proteins with intrinsically disordered regions (IDRs). In human haematological malignancies …

The expanding amyloid family: Structure, stability, function, and pathogenesis

MR Sawaya, MP Hughes, JA Rodriguez, R Riek… - Cell, 2021 - cell.com
The hidden world of amyloid biology has suddenly snapped into atomic-level focus,
revealing over 80 amyloid protein fibrils, both pathogenic and functional. Unlike globular …

Structure of cytoplasmic ring of nuclear pore complex by integrative cryo-EM and AlphaFold

P Fontana, Y Dong, X Pi, AB Tong, CW Hecksel… - Science, 2022 - science.org
INTRODUCTION The nuclear pore complex (NPC) is the molecular conduit in the nuclear
membrane of eukaryotic cells that regulates import and export of biomolecules between the …

Competing protein-RNA interaction networks control multiphase intracellular organization

DW Sanders, N Kedersha, DSW Lee, AR Strom… - Cell, 2020 - cell.com
Liquid-liquid phase separation (LLPS) mediates formation of membraneless condensates
such as those associated with RNA processing, but the rules that dictate their assembly …

The cellular environment shapes the nuclear pore complex architecture

AP Schuller, M Wojtynek, D Mankus, M Tatli… - Nature, 2021 - nature.com
Nuclear pore complexes (NPCs) create large conduits for cargo transport between the
nucleus and cytoplasm across the nuclear envelope (NE),–. These multi-megadalton …

On the role of phase separation in the biogenesis of membraneless compartments

A Musacchio - The EMBO journal, 2022 - embopress.org
Molecular mechanistic biology has ushered us into the world of life's building blocks,
revealing their interactions in macromolecular complexes and inspiring strategies for …