Review of Levan polysaccharide: From a century of past experiences to future prospects

ET Öner, L Hernández, J Combie - Biotechnology advances, 2016 - Elsevier
Levan is a fascinating β-(2, 6)-linked fructose polymer with an unusual combination of
properties characterized in this review. In nature, levan is synthesized from sucrose by a …

Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications

W Lammens, K Le Roy, L Schroeven… - Journal of …, 2009 - academic.oup.com
Glycoside hydrolases (GH) have been shown to play unique roles in various biological
processes like the biosynthesis of glycans, cell wall metabolism, plant defence, signalling …

Levan versus fructooligosaccharide synthesis using the levansucrase from Zymomonas mobilis: effect of reaction conditions

P Santos-Moriano, L Fernandez-Arrojo… - Journal of molecular …, 2015 - Elsevier
This work addresses the effect of sucrose concentration and temperature on the three
activities displayed by the levansucrase from Zymomonas mobilis: formation of levan …

A close look at the structural features and reaction conditions that modulate the synthesis of low and high molecular weight fructans by levansucrases

ME Ortiz-Soto, JR Porras-Domínguez, J Seibel… - Carbohydrate …, 2019 - Elsevier
The physicochemical properties and biological activity of levan, a generic term given to oligo-
and polysaccharides consisting of fructose units linked predominantly by β (2-6) bonds, are …

Size product modulation by enzyme concentration reveals two distinct levan elongation mechanisms in Bacillus subtilis levansucrase

E Raga-Carbajal, E Carrillo-Nava, M Costas… - …, 2016 - academic.oup.com
Two levan distributions are produced typically by Bacillus subtilis levansucrase (SacB): a
high-molecular weight (HMW) levan with an average molecular weight of 2300 kDa, and a …

Computational design of oligosaccharide producing levansucrase from Bacillus licheniformis RN-01 to improve its thermostability for production of levan-type …

M Klaewkla, R Pichyangkura… - International Journal of …, 2020 - Elsevier
Levansucrase catalyzes production of levan and levan-type fructooligosaccharides (LFOs)
with potential applications in food and pharmaceutical industries such as prebiotics and anti …

Adaptive Laboratory Evolution and Metabolic Engineering of Zymomonas mobilis for Bioethanol Production Using Molasses

J Huang, X Wang, X Chen, H Li, Y Chen… - ACS Synthetic …, 2023 - ACS Publications
Molasses with abundant sugars is widely used for bioethanol production. Although the
ethanologenic bacterium Zymomonas mobilis can use glucose, fructose, and sucrose for …

Bacillus subtilis 168 levansucrase (SacB) activity affects average levan molecular weight

JR Porras-Domínguez, Á Ávila-Fernández… - Carbohydrate …, 2015 - Elsevier
Levan is a fructan polymer that offers a variety of applications in the chemical, health,
cosmetic and food industries. Most of the levan applications depend on levan molecular …

Spectroscopic FTIR and NMR study of the interactions of sugars with proteins

M Rozenberg, S Lansky, Y Shoham… - Spectrochimica Acta Part A …, 2019 - Elsevier
FTIR and NMR spectra were measured in parallel for specific two-components mixtures of
various proteins with different sugar molecules, such as arabinose, glucose, and sucrose. In …

Kinetic and thermodynamic characteristic of Aspergillus awamori EM66 levansucrase

FA Mostafa, WAA Wahab, HA Salah… - International journal of …, 2018 - Elsevier
This study is a new trial aimed to solve levansucrase high cost and levan associated
problems during the purification process. Also, kinetic and thermodynamic study was done …