Phase transitions of associative biomacromolecules

RV Pappu, SR Cohen, F Dar, M Farag, M Kar - Chemical Reviews, 2023 - ACS Publications
Multivalent proteins and nucleic acids, collectively referred to as multivalent associative
biomacromolecules, provide the driving forces for the formation and compositional …

A conceptual framework for understanding phase separation and addressing open questions and challenges

T Mittag, RV Pappu - Molecular cell, 2022 - cell.com
Macromolecular phase separation is being recognized for its potential importance and
relevance as a driver of spatial organization within cells. Here, we describe a framework …

Synthetic protein condensates for cellular and metabolic engineering

ZG Qian, SC Huang, XX Xia - Nature chemical biology, 2022 - nature.com
Protein condensates are distinct structures assembled in living cells that concentrate
molecules via phase separation in a confined subcellular compartment. In the past decade …

Programmable viscoelasticity in protein-RNA condensates with disordered sticker-spacer polypeptides

I Alshareedah, MM Moosa, M Pham… - Nature …, 2021 - nature.com
Liquid-liquid phase separation of multivalent proteins and RNAs drives the formation of
biomolecular condensates that facilitate membrane-free compartmentalization of subcellular …

Sequence-dependent material properties of biomolecular condensates and their relation to dilute phase conformations

D Sundaravadivelu Devarajan, J Wang… - Nature …, 2024 - nature.com
Material properties of phase-separated biomolecular condensates, enriched with disordered
proteins, dictate many cellular functions. Contrary to the progress made in understanding the …

Droplet physics and intracellular phase separation

F Jülicher, CA Weber - Annual Review of Condensed Matter …, 2024 - annualreviews.org
Living cells are spatially organized by compartments that can nucleate, grow, and dissolve.
Compartmentalization can emerge by phase separation, leading to the formation of droplets …

Sequence-specific interactions determine viscoelasticity and ageing dynamics of protein condensates

I Alshareedah, WM Borcherds, SR Cohen, A Singh… - Nature Physics, 2024 - nature.com
Biomolecular condensates are viscoelastic materials. Here we investigate the determinants
of the sequence-encoded and age-dependent viscoelasticity of condensates formed by the …

Adenosine triphosphate mediates phase separation of disordered basic proteins by bridging intermolecular interaction networks

D Kota, R Prasad, HX Zhou - Journal of the American Chemical …, 2024 - ACS Publications
Adenosine triphosphate (ATP) is an abundant molecule with crucial cellular roles as the
energy currency and a building block of nucleic acids and for protein phosphorylation. Here …

Fundamental Aspects of Phase-Separated Biomolecular Condensates

HX Zhou, D Kota, S Qin, R Prasad - Chemical Reviews, 2024 - ACS Publications
Biomolecular condensates, formed through phase separation, are upending our
understanding in much of molecular, cell, and developmental biology. There is an urgent …

Biomolecular condensates form spatially inhomogeneous network fluids

F Dar, SR Cohen, DM Mitrea, AH Phillips… - Nature …, 2024 - nature.com
The functions of biomolecular condensates are thought to be influenced by their material
properties, and these will be determined by the internal organization of molecules within …