Solid-state NMR investigations of extracellular matrixes and cell walls of algae, bacteria, fungi, and plants

N Ghassemi, A Poulhazan, F Deligey… - Chemical …, 2021 - ACS Publications
Extracellular matrixes (ECMs), such as the cell walls and biofilms, are important for
supporting cell integrity and function and regulating intercellular communication. These …

Dynamic nuclear polarization for sensitivity enhancement in biomolecular solid-state NMR

T Biedenbänder, V Aladin, S Saeidpour… - Chemical …, 2022 - ACS Publications
Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

Advanced techniques for detecting protein misfolding and aggregation in cellular environments

Y Bai, S Zhang, H Dong, Y Liu, C Liu… - Chemical Reviews, 2023 - ACS Publications
Protein misfolding and aggregation, a key contributor to the progression of numerous
neurodegenerative diseases, results in functional deficiencies and the creation of harmful …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

Impact of cellular crowding on protein structural dynamics investigated by EPR spectroscopy

A Pierro, A Bonucci, A Magalon, V Belle… - Chemical …, 2024 - ACS Publications
The study of how the intracellular medium influences protein structural dynamics and protein–
protein interactions is a captivating area of research for scientists aiming to comprehend …

Highly bioresistant, hydrophilic and rigidly linked trityl-nitroxide biradicals for cellular high-field dynamic nuclear polarization

R Yao, D Beriashvili, W Zhang, S Li, A Safeer… - Chemical …, 2022 - pubs.rsc.org
Cellular dynamic nuclear polarization (DNP) has been an effective means of overcoming the
intrinsic sensitivity limitations of solid-state nuclear magnetic resonance (ssNMR) …

Polarizing agents for efficient high field DNP solid-state NMR spectroscopy under magic-angle spinning: from design principles to formulation strategies

G Menzildjian, J Schlagnitweit, G Casano, O Ouari… - Chemical …, 2023 - pubs.rsc.org
Dynamic Nuclear Polarization (DNP) has recently emerged as a cornerstone approach to
enhance the sensitivity of solid-state NMR spectroscopy under Magic Angle Spinning (MAS) …

A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity

D Beriashvili, R Yao, F D'Amico, M Krafčíková… - Chemical …, 2023 - pubs.rsc.org
Studying the structural aspects of proteins within sub-cellular compartments is of growing
interest. Dynamic nuclear polarization supported solid-state NMR (DNP-ssNMR) is uniquely …

Gd3+–Trityl–Nitroxide Triple Labeling and Distance Measurements in the Heterooligomeric Cobalamin Transport Complex in the Native Lipid Bilayers

S Ketter, B Joseph - Journal of the American Chemical Society, 2023 - ACS Publications
Increased efforts are being made for observing proteins in their native environments. Pulsed
electron–electron double resonance spectroscopy (PELDOR, also known as DEER) is a …

PyrroTriPol: a semi-rigid trityl-nitroxide for high field dynamic nuclear polarization

T Halbritter, R Harrabi, S Paul, J van Tol, D Lee… - Chemical …, 2023 - pubs.rsc.org
Magic angle spinning (MAS) dynamic nuclear polarization (DNP) has significantly
broadened the scope of solid-state NMR to study biomolecular systems and materials. In …