Physical principles underlying the complex biology of intracellular phase transitions

JM Choi, AS Holehouse… - Annual review of …, 2020 - annualreviews.org
Many biomolecular condensates appear to form via spontaneous or driven processes that
have the hallmarks of intracellular phase transitions. This suggests that a common …

[HTML][HTML] Considerations and challenges in studying liquid-liquid phase separation and biomolecular condensates

S Alberti, A Gladfelter, T Mittag - Cell, 2019 - cell.com
Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation
of membraneless compartments in cells. This realization has motivated major efforts to …

Phase separation of protein mixtures is driven by the interplay of homotypic and heterotypic interactions

M Farag, WM Borcherds, A Bremer, T Mittag… - Nature …, 2023 - nature.com
Prion-like low-complexity domains (PLCDs) are involved in the formation and regulation of
distinct biomolecular condensates that form via phase separation coupled to percolation …

Condensates formed by prion-like low-complexity domains have small-world network structures and interfaces defined by expanded conformations

M Farag, SR Cohen, WM Borcherds, A Bremer… - Nature …, 2022 - nature.com
Biomolecular condensates form via coupled associative and segregative phase transitions
of multivalent associative macromolecules. Phase separation coupled to percolation is one …

Physics-driven coarse-grained model for biomolecular phase separation with near-quantitative accuracy

JA Joseph, A Reinhardt, A Aguirre, PY Chew… - Nature Computational …, 2021 - nature.com
Various physics-and data-driven sequence-dependent protein coarse-grained models have
been developed to study biomolecular phase separation and elucidate the dominant …

Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains

A Bremer, M Farag, WM Borcherds, I Peran… - Nature …, 2022 - nature.com
Prion-like low-complexity domains (PLCDs) have distinctive sequence grammars that
determine their driving forces for phase separation. Here we uncover the physicochemical …

Intermolecular interactions underlie protein/peptide phase separation irrespective of sequence and structure at crowded milieu

M Poudyal, K Patel, L Gadhe, AS Sawner… - Nature …, 2023 - nature.com
Liquid-liquid phase separation (LLPS) has emerged as a crucial biological phenomenon
underlying the sequestration of macromolecules (such as proteins and nucleic acids) into …

Intrinsically disordered linkers determine the interplay between phase separation and gelation in multivalent proteins

TS Harmon, AS Holehouse, MK Rosen, RV Pappu - elife, 2017 - elifesciences.org
Phase transitions of linear multivalent proteins control the reversible formation of many
intracellular membraneless bodies. Specific non-covalent crosslinks involving …

Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation

JP Brady, PJ Farber, A Sekhar, YH Lin… - Proceedings of the …, 2017 - National Acad Sciences
Membrane encapsulation is frequently used by the cell to sequester biomolecules and
compartmentalize their function. Cells also concentrate molecules into phase-separated …

LASSI: A lattice model for simulating phase transitions of multivalent proteins

JM Choi, F Dar, RV Pappu - PLoS computational biology, 2019 - journals.plos.org
Many biomolecular condensates form via spontaneous phase transitions that are driven by
multivalent proteins. These molecules are biological instantiations of associative polymers …