Reaction Mechanisms in Carbohydrate-Active Enzymes: Glycoside Hydrolases and Glycosyltransferases. Insights from ab Initio Quantum Mechanics/Molecular …

A Ardèvol, C Rovira - Journal of the American Chemical Society, 2015 - ACS Publications
Carbohydrate-active enzymes such as glycoside hydrolases (GHs) and glycosyltransferases
(GTs) are of growing importance as drug targets. The development of efficient competitive …

Thermostable enzymes as biocatalysts in the biofuel industry

CJ Yeoman, Y Han, D Dodd, CM Schroeder… - Advances in applied …, 2010 - Elsevier
Lignocellulose is the most abundant carbohydrate source in nature and represents an ideal
renewable energy source. Thermostable enzymes that hydrolyze lignocellulose to its …

Conformational analyses of the reaction coordinate of glycosidases

GJ Davies, A Planas, C Rovira - Accounts of chemical research, 2012 - ACS Publications
The enzymatic hydrolysis of the glycosidic bond is catalyzed by diverse enzymes generically
termed glycoside hydrolases (hereafter GHs) or glycosidases. The many sequence-based …

Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases

CS Park, MH Yoo, KH Noh, DK Oh - Applied microbiology and …, 2010 - Springer
Ginsenosides are the principal components responsible for the pharmaceutical activities of
ginseng. The minor ginsenosides, which are also pharmaceutically active, can be produced …

β-Xylosidases and α-l-arabinofuranosidases: accessory enzymes for arabinoxylan degradation

S Lagaert, A Pollet, CM Courtin, G Volckaert - Biotechnology advances, 2014 - Elsevier
Arabinoxylan (AX) is among the most abundant hemicelluloses on earth and one of the
major components of feedstocks that are currently investigated as a source for advanced …

How sugars pucker: electronic structure calculations map the kinetic landscape of five biologically paramount monosaccharides and their implications for enzymatic …

HB Mayes, LJ Broadbelt… - Journal of the American …, 2014 - ACS Publications
Glycoside hydrolases (GHs) distort carbohydrate ring geometry along particular “catalytic
itineraries” during the cleavage of glycosidic bonds, illustrating the relationship between …

The structure of an inverting GH43 β-xylosidase from Geobacillus stearothermophilus with its substrate reveals the role of the three catalytic residues

C Brüx, A Ben-David, D Shallom-Shezifi, M Leon… - Journal of molecular …, 2006 - Elsevier
β-d-Xylosidases are glycoside hydrolases that catalyze the release of xylose units from short
xylooligosaccharides and are engaged in the final breakdown of plant cell-wall …

Characterization of the Pseudomonas aeruginosa glycoside hydrolase PslG reveals that its levels are critical for Psl polysaccharide biosynthesis and biofilm formation

P Baker, GB Whitfield, PJ Hill, DJ Little… - Journal of Biological …, 2015 - ASBMB
A key component of colonization, biofilm formation, and protection of the opportunistic
human pathogen Pseudomonas aeruginosa is the biosynthesis of the exopolysaccharide …

β-Xylosidases: Structural diversity, catalytic mechanism, and inhibition by monosaccharides

A Rohman, BW Dijkstra, NNT Puspaningsih - International Journal of …, 2019 - mdpi.com
Xylan, a prominent component of cellulosic biomass, has a high potential for degradation
into reducing sugars, and subsequent conversion into bioethanol. This process requires a …

[HTML][HTML] Glycosidase mechanisms: Sugar conformations and reactivity in endo-and exo-acting enzymes

MAB Morais, A Nin-Hill, C Rovira - Current Opinion in Chemical Biology, 2023 - Elsevier
The enzymatic breakdown of carbohydrates plays a critical role in several biological events
and enables the development of sustainable processes to obtain bioproducts and biofuels …