Cell culture in microfluidic droplets
Cell manipulation in droplets has emerged as one of the great successes of microfluidic
technologies, with the development of single-cell screening. However, the droplet format has …
technologies, with the development of single-cell screening. However, the droplet format has …
Comprehensive evaluation of organotypic and microphysiological liver models for prediction of drug-induced liver injury
Drug-induced liver injury (DILI) is a major concern for the pharmaceutical industry and
constitutes one of the most important reasons for the termination of promising drug …
constitutes one of the most important reasons for the termination of promising drug …
Current strategies with implementation of three-dimensional cell culture: the challenge of quantification
J Temple, E Velliou, M Shehata, R Lévy… - Interface …, 2022 - royalsocietypublishing.org
From growing cells in spheroids to arranging them on complex engineered scaffolds, three-
dimensional cell culture protocols are rapidly expanding and diversifying. While these …
dimensional cell culture protocols are rapidly expanding and diversifying. While these …
Advanced biomedical applications based on emerging 3D cell culturing platforms
It is of great value to develop reliable in vitro models for cell biology and toxicology.
However, ethical issues and the decreasing number of donors restrict the further use of …
However, ethical issues and the decreasing number of donors restrict the further use of …
Multiscale modelling of drug transport and metabolism in liver spheroids
JA Leedale, JA Kyffin, AL Harding… - Interface …, 2020 - royalsocietypublishing.org
In early preclinical drug development, potential candidates are tested in the laboratory using
isolated cells. These in vitro experiments traditionally involve cells cultured in a two …
isolated cells. These in vitro experiments traditionally involve cells cultured in a two …
3D bioprinting of dECM-incorporated hepatocyte spheroid for simultaneous promotion of cell-cell and-ECM interactions
The cell spheroid technology, which greatly enhances cell-cell interactions, has gained
significant attention in the development of in vitro liver models. However, existing cell …
significant attention in the development of in vitro liver models. However, existing cell …
3D spheroids of human primary urine-derived stem cells in the assessment of drug-induced mitochondrial toxicity
H Ding, K Jambunathan, G Jiang, DM Margolis, I Leng… - Pharmaceutics, 2022 - mdpi.com
Mitochondrial toxicity (Mito-Tox) risk has increased due to the administration of several
classes of drugs, particularly some life-long antiretroviral drugs for HIV+ individuals …
classes of drugs, particularly some life-long antiretroviral drugs for HIV+ individuals …
The native liver as inspiration to create superior in vitro hepatic models
N Carpentier, L Urbani, P Dubruel… - Biomaterials …, 2023 - pubs.rsc.org
Drug induced liver injury (DILI) is one of the major reasons of drug withdrawal during the
different phases of drug development. The later in the drug development a drug is …
different phases of drug development. The later in the drug development a drug is …
Culture of cancer spheroids and evaluation of anti-cancer drugs in 3D-printed miniaturized continuous stirred tank reactors (mCSTRs)
S Gallegos-Martínez, IM Lara-Mayorga… - …, 2022 - iopscience.iop.org
Cancer continues to be a leading cause of mortality in modern societies; therefore, improved
and more reliable in vitro cancer models are needed to expedite fundamental research and …
and more reliable in vitro cancer models are needed to expedite fundamental research and …
The choice of ultra‐low attachment plates impacts primary human and primary canine hepatocyte spheroid formation, phenotypes, and function
C Xing, A Kemas, E Mickols, K Klein… - Biotechnology …, 2024 - Wiley Online Library
Organotypic three‐dimensional liver spheroid cultures in which hepatic cells retain their
molecular phenotype and functionality have emerged as powerful tools for preclinical drug …
molecular phenotype and functionality have emerged as powerful tools for preclinical drug …