BACKGROUND Cellular life depends on the ability of organisms to accurately duplicate and
transmit genetic information between generations. In bacteria, archaea, and eukaryotes, this …

The initiation of DNA replication represents a committing step to cell proliferation.
Appropriate replication onset depends on multiprotein complexes that help properly …

Hexameric helicases couple ATP hydrolysis to processive separation of nucleic acid
duplexes, a process critical for gene expression, DNA replication, and repair. All hexameric …

Loading of the replicative DNA helicase at origins of replication is of central importance in
DNA replication. As the first of the replication fork proteins assemble at chromosomal origins …

New antibiotics with novel modes of action are required to combat the growing threat posed
by multi-drug resistant bacteria. Over the last decade, genome sequencing and other high …

Dedicated AAA+ ATPases deposit hexameric ring-shaped helicases onto DNA to promote
replication in cellular organisms. To understand how loading occurs, we used electron …

In cells, dedicated AAA+ ATPases deposit hexameric, ring-shaped helicases onto DNA to
initiate chromosomal replication. To better understand the mechanisms by which helicase …

DnaB helicases are motor proteins that couple ATP-hydrolysis to the loading of the protein
onto DNA at the replication fork and to translocation along DNA to separate double-stranded …

Summary An AAA+ ATPase, DnaC, delivers DnaB helicase at the E. coli chromosomal origin
by a poorly understood process. This report shows that mutant proteins bearing alanine …

The initiation of chromosomal DNA replication starts at a replication origin, which in bacteria
is a discrete locus that contains DNA sequence motifs recognized by an initiator protein …