Locking the GFP Fluorophore to Enhance Its Emission Intensity

JRM Ferreira, CIC Esteves, MMB Marques, S Guieu - Molecules, 2022 - mdpi.com
The Green Fluorescent Protein (GFP) and its analogues have been widely used as
fluorescent biomarkers in cell biology. Yet, the chromophore responsible for the …

Fluorescence Amplification of Unsaturated Oxazolones Using Palladium: Photophysical and Computational Studies

D Dalmau, O Crespo, JM Matxain… - Inorganic …, 2023 - ACS Publications
Weakly fluorescent (Z)-4-arylidene-5-(4 H)-oxazolones (1), ΦPL< 0.1%, containing a variety
of conjugated aromatic fragments and/or charged arylidene moieties, have been …

Fluorescent Orthopalladated Complexes of 4-Aryliden-5(4H)-oxazolones from the Kaede Protein: Synthesis and Characterization

E Laga, D Dalmau, S Arregui, O Crespo, AI Jimenez… - Molecules, 2021 - mdpi.com
The goal of the work reported here was to amplify the fluorescent properties of 4-aryliden-5
(4 H)-oxazolones by suppression of the hula-twist non-radiative deactivation pathway. This …

The Role of N‐Substituents in Radiationless Deactivation of Aminated Derivatives of a Locked GFP Chromophore

NS Baleeva, SO Zaitseva… - European Journal of …, 2017 - Wiley Online Library
We report the creation of a novel group of the ABDI‐BF2 fluorescent dyes based on the
conformationally locked GFP chromophore. We studied the intramolecular mechanism of …

Orthopalladation of GFP‐Like Fluorophores Through C–H Bond Activation: Scope and Photophysical Properties

S Collado, A Pueyo, C Baudequin… - European Journal of …, 2018 - Wiley Online Library
The luminescence of oxazolones R1‐C6H4CH= CC (O) O‐CN (R2)(1a–1j) and
imidazolones R1‐C6H4CH= CC (O) NR3CN (R2)(1k–1q) has been examined. The new …

A hybrid molecule of a GFP chromophore analogue and cholestene as a viscosity-dependent and cholesterol-responsive fluorescent sensor

M Ikejiri, K Mori, R Miyagi, R Konishi… - Organic & …, 2017 - pubs.rsc.org
Diarylmethylenated and cholestene (or-tane)-hybrid analogues of the GFP chromophore,
namely, Ch-DAINs were successfully synthesised by a condensation reaction between …

Designing red-shifted molecular emitters based on the annulated locked GFP chromophore derivatives

GD Sinenko, DA Farkhutdinova… - International Journal of …, 2021 - mdpi.com
Bioimaging techniques require development of a wide variety of fluorescent probes that
absorb and emit red light. One way to shift absorption and emission of a chromophore to …

Fluorescence Decay Analysis of the Model Compounds as an Approach to Photophysical Engineering of Fluorescent Proteins

NS Baleeva, MS Baranov, AM Bogdanov - Russian Journal of Bioorganic …, 2023 - Springer
The study of structure–function relationships between a chromophore and its protein
environment plays a key role in photophysical engineering of fluorescent proteins (FPs) …

Synthesis of 2-arylidene-6,7-dihydroimidazo[1,2-a]pyrazine-3,8(2H,5H)-diones by oxidation of 4-arylidene-2-methyl-1H-imidazol-5(4H)-ones with selenium …

NS Baleeva, EA Levina, MS Baranov - Chemistry of Heterocyclic …, 2017 - Springer
(Z)-4-Arylidene-2-methyl-1 H-imidazol-5 (4 H)-ones containing a substituted aminoethyl
group at the N-1 atom can be oxidized with an excess of selenium dioxide, resulting in the …

The Sonogashira reaction as a new method for the modification of borated analogues of the green fluorescence protein chromophore

NS Baleeva, MS Baranov - Russian Journal of Bioorganic Chemistry, 2017 - Springer
The Sonogashira reaction was used for modifications of borated green fluorescence protein
chromophore derivatives, 4-(2-(difluoroboryl) benzylidene)-1 H-imidazol-5 (4 H)-ones, for …