Dynamic culture of mesenchymal stromal/stem cell spheroids and secretion of paracrine factors

P Fuentes, MJ Torres, R Arancibia… - … in Bioengineering and …, 2022 - frontiersin.org
In recent years, conditioned medium (CM) obtained from the culture of mesenchymal
stromal/stem cells (MSCs) has been shown to effectively promote tissue repair and modulate …

Bioengineered living bone grafts—A concise review on bioreactors and production techniques in vitro

P Kazimierczak, A Przekora - International journal of molecular sciences, 2022 - mdpi.com
It has been observed that bone fractures carry a risk of high mortality and morbidity. The
deployment of a proper bone healing method is essential to achieve the desired success …

Biological and Mechanical Response of Graphene Oxide Surface‐Treated Polylactic Acid 3D‐Printed Bone Scaffolds: Experimental and Numerical Approaches

M Mashhadi Keshtiban, H Taghvaei… - Advanced …, 2024 - Wiley Online Library
Employing 3D printing bone scaffolds with various polymers is growing due to their
biocompatibility, biodegradability, and good mechanical properties. However, their …

Induction of osteogenic differentiation of bone marrow stromal cells on 3D polyester-based scaffolds solely by subphysiological fluidic stimulation in a laminar flow …

S Yamada, MA Yassin, T Schwarz… - Journal of tissue …, 2021 - journals.sagepub.com
The fatal determination of bone marrow mesenchymal stem/stromal cells (BMSC) is closely
associated with mechano-environmental factors in addition to biochemical clues. The aim of …

Hep3Gel: a shape-shifting extracellular matrix-based, three-dimensional liver model adaptable to different culture systems

G Guagliano, C Volpini, L Sardelli… - ACS biomaterials …, 2022 - ACS Publications
Drug-induced hepatotoxicity is a leading cause of clinical trial withdrawal. Therefore, in vitro
modeling the hepatic behavior and functionalities is not only crucial to better understand …

Toward 3D‐Bioprinted Models of the Liver to Boost Drug Development

G Guagliano, C Volpini… - Macromolecular …, 2022 - Wiley Online Library
The main problems in drug development are connected to enormous costs related to the
paltry success rate. The current situation empowered the development of high‐throughput …

Optimization and validation of a custom-designed perfusion bioreactor for bone tissue engineering: Flow assessment and optimal culture environmental conditions

S Yamada, MA Yassin, T Schwarz, K Mustafa… - … in Bioengineering and …, 2022 - frontiersin.org
Various perfusion bioreactor systems have been designed to improve cell culture with three-
dimensional porous scaffolds, and there is some evidence that fluid force improves the …

Dynamic process enhancement on chitosan/gelatin/nano-hydroxyapatite-bone derived multilayer scaffold for osteochondral tissue repair

X Hu, S Zheng, R Zhang, Y Wang, Z Jiao, W Li… - Biomaterials …, 2022 - Elsevier
Accurate repair of osteochondral defects is a great challenge due to the complex structure of
osteochondral defects. The current study aims to prepare a biomimetic osteochondral …

Bioreactor expansion reconfigures metabolism and extracellular vesicle biogenesis of human adipose-derived stem cells in vitro

R Jeske, X Chen, S Ma, EZ Zeng, T Driscoll… - Biochemical engineering …, 2022 - Elsevier
Human mesenchymal stem cells (hMSCs), including human adipose tissue-derived stem
cells (hASCs), as well as the secreted extracellular vesicles (EVs), are promising …

Clarifying the tooth-derived stem cells behavior in a 3D biomimetic scaffold for bone tissue engineering applications

CL Salgado, CC Barrias, FJM Monteiro - Frontiers in bioengineering …, 2020 - frontiersin.org
Massive amounts of cell are needed for creating tissue engineered 3D constructs, which
often requires culture on scaffolds under dynamic conditions to facilitate nutrients and …