[HTML][HTML] Revolutionizing biofuel generation: Unleashing the power of CRISPR-Cas mediated gene editing of extremophiles

D Garg, MK Samota, N Kontis, N Patel, S Bala… - Microbiological …, 2023 - Elsevier
Molecular biology techniques like gene editing have altered the specific genes in micro-
organisms to increase their efficiency to produce biofuels. This review paper investigates the …

Self-assembly systems to troubleshoot metabolic engineering challenges

Z Chen, T Wu, S Yu, M Li, X Fan, YX Huo - Trends in Biotechnology, 2024 - cell.com
Enzyme self-assembly is a technology in which enzyme units can aggregate into ordered
macromolecules, assisted by scaffolds. In metabolic engineering, self-assembly strategies …

Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9

J Gao, J Xu, Y Zuo, C Ye, L Jiang, L Feng… - ACS Synthetic …, 2022 - ACS Publications
Pichia pastoris, an important methylotrophic yeast, is currently mainly used for the
expression of recombinant proteins and has great potential applications in the production of …

Rewiring yeast metabolism for producing 2, 3-butanediol and two downstream applications: Techno-economic analysis and life cycle assessment of methyl ethyl …

JW Lee, SS Bhagwat, N Kuanyshev, YB Cho… - Chemical Engineering …, 2023 - Elsevier
Rising concerns for sustainability and global climate change have driven the development of
sustainable production pathways for biofuels and chemicals from lignocellulosic biomass via …

Metabolic engineering of non-pathogenic microorganisms for 2, 3-butanediol production

JW Lee, YG Lee, YS Jin, CV Rao - Applied Microbiology and …, 2021 - Springer
Butanediol (2, 3-BDO) is a promising commodity chemical with various industrial
applications. While petroleum-based chemical processes currently dominate the industrial …

Regulation of carbon flux and NADH/NAD+ supply to enhance 2, 3-butanediol production in Enterobacter aerogenes

P Lu, T Gao, R Bai, J Yang, Y Xu, W Chu, K Jiang… - Journal of …, 2022 - Elsevier
As a valuable platform chemical, 2, 3-Butanediol (2, 3-BDO) has a variety of industrial
applications, and its microbial production is particularly attractive as an alternative to …

Screening low-methanol and high-aroma produced yeasts for cider fermentation by transcriptive characterization

L Liu, PT Zhao, CY Hu, D Tian, H Deng… - Frontiers in …, 2022 - frontiersin.org
The commercial active dry yeast strains used for cider production in China are far behind the
requirements of the cider industry development in recent decades. In this study, eight yeasts …

Biotechnological production of chiral acetoin

W Meng, C Ma, P Xu, C Gao - Trends in Biotechnology, 2022 - cell.com
Acetoin (AC) is an important platform bulk chemical with versatile applications. It exists in
two stereoisomeric forms:(3R)-AC and (3S)-AC. Both stereoisomers could be potentially …

Development of an industrial yeast strain for efficient production of 2, 3-butanediol

G Huo, MR Foulquié-Moreno, JM Thevelein - Microbial Cell Factories, 2022 - Springer
As part of the transition from a fossil resources-based economy to a bio-based economy, the
production of platform chemicals by microbial cell factories has gained strong interest. 2, 3 …

Efficient acetoin production from pyruvate by engineered Halomonas bluephagenesis whole-cell biocatalysis

M Zheng, Z Cui, J Zhang, J Fu, Z Wang… - Frontiers of Chemical …, 2023 - Springer
Acetoin is an important platform chemical, which has a wide range of applications in many
industries. Halomonas bluephagenesis, a chassis for next generation of industrial …