Agrobacterium rhizogenes-mediated transformation: root cultures as a source of alkaloids

N Sevón, KM Oksman-Caldentey - Planta Medica, 2002 - thieme-connect.com
Hairy roots, transformed with Agrobacterium rhizogenes, have been found to be suitable for
the production of secondary metabolites because of their stable and high productivity in …

Large-scale plant cell culture

SC Roberts, ML Shuler - Current opinion in Biotechnology, 1997 - Elsevier
Progress towards the commercial-scale use of plant cell cultures over the past three years
has been significant. Elicitation, particularly with methyl jasmonate, has been effective at …

The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida

SI Pitta–Alvarez, TC Spollansky, AM Giulietti - Enzyme and microbial …, 2000 - Elsevier
Hairy root cultures of Brugmansia candida produce the tropane alkaloids scopolamine and
hyoscyamine. In an attempt to increase productivity, several biotic and abiotic elicitors were …

Effect of Elicitor Dosage and Exposure Time on Biosynthesis of Indole Alkaloids by Catharanthus roseus Hairy Root Cultures

SK Rijhwani, JV Shanks - Biotechnology progress, 1998 - Wiley Online Library
Late exponential phase hairy root cultures of Catharanthus roseus were elicited with
pectinase and jasmonic acid. The effects of elicitor concentration and exposure time on …

Agrobacterium rhizogenes‐mediated transformation of opium poppy, Papaver somniferum L., and California poppy, Eschscholzia californica Cham., root cultures

SU Park, PJ Facchini - Journal of Experimental Botany, 2000 - academic.oup.com
An efficient protocol for the establishment of transgenic opium poppy (Papaver somniferum
L.) and California poppy (Eschscholzia californica Cham.) root cultures using Agrobacterium …

Screening of Catharanthus roseus secondary metabolites by high-performance liquid chromatography

C Tikhomiroff, M Jolicoeur - journal of chromatography A, 2002 - Elsevier
Two direct HPLC analytical methods for the screening of the major indole alkaloids of
Catharanthus roseus hairy roots and their iridoid precursors have been developed …

Biosynthesis of terpenoid indole alkaloids in Catharanthus roseus cells

R Verpoorte, R van der Heijden… - The alkaloids: Chemistry …, 1997 - Elsevier
Publisher Summary This chapter studies the biosynthesis of the terpenoid indole alkaloids in
C. roseus. On the level of the enzymes, certain steps have now been well characterized, but …

Elicitation of different Panax ginseng transformed root phenotypes for an improved ginsenoside production

J Palazón, RM Cusidó, M Bonfill, A Mallol… - Plant Physiology and …, 2003 - Elsevier
We tested the effect of the presence in the culture medium of chitosan, vanadyl sulfate or
methyl jasmonate on growth and ginsenoside production of three stable hairy root lines of …

Hairy root cultures for secondary metabolites production

L Pistelli, A Giovannini, B Ruffoni, A Bertoli… - Bio-farms for …, 2010 - Springer
Hairy roots (HRs) are differentiated cultures of transformed roots generated by the infection
of wounded higher plants with Agrobacterium rhizogenes. This pathogen causes the HR …

Transcriptome analysis of Arabidopsis roots treated with signaling compounds: a focus on signal transduction, metabolic regulation and secretion

DV Badri, VM Loyola‐Vargas, J Du… - New …, 2008 - Wiley Online Library
Gene expression in response to signaling molecules has been well studied in the leaves of
the model plant species Arabidopsis thaliana. However, knowledge of gene expression and …