Single-cell profiling methods have had a profound impact on the understanding of cellular
heterogeneity. While genomes and transcriptomes can be explored at the single-cell level …

Despite tremendous gains over the past decade, methods for characterizing proteins have
generally lagged behind those for nucleic acids, which are characterized by extremely high …

Proteomic analysis can be a critical bottleneck in cellular characterization. The current
paradigm relies primarily on mass spectrometry of peptides and affinity reagents (ie …

Proteins have been considered key building blocks of life. In particular, the protein content of
an organism and a cell offers significant information for the in-depth understanding of the …

Proteins are the primary functional actors of the cell. While proteoform diversity is known to
be highly biologically relevant, current protein analysis methods are of limited use for …

Electrolyte-gated silicon field-effect transistors (FETs) capable of detecting single molecules
could enable high-throughput molecular sensing chips to advance, for example, genomics …

While mass spectrometry still dominates proteomics research, alternative and potentially
disruptive, next-generation technologies are receiving increased investment and attention …

Bottom-up proteomic experiments often require selective conjugation or labeling of the N-
and/or C-termini of peptides resulting from proteolytic digestion. For example, techniques …

A binary/digital method is proposed in theory for the identification of single amino acids
(AAs) in the bulk or with a few molecules from a single binary measurement. Combined with …

Recent advances in DNA sequencing methods revolutionized biology by providing highly
accurate reads, with high throughput or high read length. These read data are being used in …