Full-length recombinant antibodies from Escherichia coli: production, characterization, effector function (Fc) engineering, and clinical evaluation
MH Rashid - MAbs, 2022 - Taylor & Francis
Although several antibody fragments and antibody fragment-fusion proteins produced in
Escherichia coli (E. coli) are approved as therapeutics for various human diseases, a full …
Escherichia coli (E. coli) are approved as therapeutics for various human diseases, a full …
Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coli
AA Tungekar, LW Ruddock - Scientific Reports, 2023 - nature.com
With increased accessibility and tissue penetration, smaller antibody formats such as
antibody fragments (Fab) and single chain variable fragments (scFv) show potential as …
antibody fragments (Fab) and single chain variable fragments (scFv) show potential as …
QbD Based Media Development for the Production of Fab Fragments in E. coli
D Kumar, J Batra, C Komives, AS Rathore - Bioengineering, 2019 - mdpi.com
Ranibizumab is a biotherapeutic Fab fragment used for the treatment of age-related macular
degeneration and macular oedema. It is currently expressed in the gram-negative …
degeneration and macular oedema. It is currently expressed in the gram-negative …
[HTML][HTML] Approaches for high-throughput quantification of periplasmic recombinant proteins
A Osgerby, TW Overton - New Biotechnology, 2023 - Elsevier
The Gram-negative periplasm is a convenient location for the accumulation of many
recombinant proteins including biopharmaceutical products. It is the site of disulphide bond …
recombinant proteins including biopharmaceutical products. It is the site of disulphide bond …
Cloning and expression of antibody fragment (Fab) II: Effect of expression hosts on light and heavy chain gene expression
D Mehta, T Chirmade, N Nainwal… - Biochemical Engineering …, 2023 - Elsevier
Protein expression stoichiometry of individual subunits plays a critical role in the formation of
multi-domain functional therapeutic proteins like antibody fragments. In this study, we have …
multi-domain functional therapeutic proteins like antibody fragments. In this study, we have …
Evaluation of primary capture procedures for recombinant antibodies manufactured in Pichia pastoris: method, viability and economics
G Bhavsar - 2023 - discovery.ucl.ac.uk
The eukaryotic yeast Pichia pastoris is an increasingly applied platform for heterologous
protein production for therapeutic and diagnostic purposes. Recent advances in high …
protein production for therapeutic and diagnostic purposes. Recent advances in high …
Understanding the transcriptional landscape of Chinese hamster ovary (CHO) cell lines using next-generation sequencing technology, RNA-seq, under industrially …
YK Gowtham - 2016 - search.proquest.com
Recombinant therapeutic proteins are widely used to treat life-threatening diseases
including cancer. Chinese hamster ovary (CHO) cells have been used for over 25 years in …
including cancer. Chinese hamster ovary (CHO) cells have been used for over 25 years in …
Drugs of the Future-Bispecific Antibodies: An investigaion of future development needs
CF Svahn, A Khan, A Wahlsten, T Larsson, T Koivula… - 2019 - diva-portal.org
This report reviews the field of bispecific antibodies, artificially engineered antibodies that
have the ability to bind two or more different antigen simultaneously. Historical as well as …
have the ability to bind two or more different antigen simultaneously. Historical as well as …