Autophagy: assays and artifacts

S Barth, D Glick, KF Macleod - The Journal of pathology, 2010 - Wiley Online Library
Autophagy is a fundamental and phylogenetically conserved self‐degradation process that
is characterized by the formation of double‐layered vesicles (autophagosomes) around …

[PDF][PDF] Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

DJ Klionsky, H Abeliovich, P Agostinis, DK Agrawal… - Autophagy, 2008 - Taylor & Francis
Research in autophagy continues to accelerate, 1 and as a result many new scientists are
entering the field. Accordingly, it is important to establish a standard set of criteria for …

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1

DJ Klionsky, AK Abdel-Aziz, S Abdelfatah, M Abdellatif… - autophagy, 2021 - Taylor & Francis
In 2008, we published the first set of guidelines for standardizing research in autophagy.
Since then, this topic has received increasing attention, and many scientists have entered …

Autophagy proteins regulate ERK phosphorylation

N Martinez-Lopez, D Athonvarangkul, P Mishall… - Nature …, 2013 - nature.com
Autophagy is a conserved pathway that maintains cellular quality control. Extracellular
signal-regulated kinase (ERK) controls various aspects of cell physiology including …

Autophagosome proteins LC3A, LC3B and LC3C have distinct subcellular distribution kinetics and expression in cancer cell lines

MI Koukourakis, D Kalamida, A Giatromanolaki… - PloS one, 2015 - journals.plos.org
LC3s (MAP1-LC3A, B and C) are structural proteins of autophagosomal membranes, widely
used as biomarkers of autophagy. Whether these three LC3 proteins have a similar …

Endoplasmic reticulum stress and autophagy in the pathogenesis of non-alcoholic fatty liver disease (NAFLD): current evidence and perspectives

CM Flessa, I Kyrou, N Nasiri-Ansari, G Kaltsas… - Current obesity …, 2021 - Springer
Abstract Purpose of Review Non-alcoholic fatty liver disease (NAFLD) is one of the most
common causes of chronic liver disease with rising prevalence worldwide. Herein, we …

Autophagy in tumor suppression and cancer therapy

CP Kung, A Budina, G Balaburski… - Critical Reviews™ in …, 2011 - dl.begellhouse.com
Autophagy is a stress-induced cell survival program whereby cells under metabolic,
proteotoxic, or other stress remove dysfunctional organelles and/or misfolded …

Platelet-membrane-camouflaged black phosphorus quantum dots enhance anticancer effect mediated by apoptosis and autophagy

Y Shang, Q Wang, B Wu, Q Zhao, J Li… - … applied materials & …, 2019 - ACS Publications
Hederagenin (HED) has poor anticancer activity whose mechanism remains unclear and
unsystematic. Free drugs for cancer treatment exhibit disadvantages such as poor targeting …

Characterization of macroautophagic flux in vivo using a leupeptin-based assay

J Haspel, RS Shaik, E Ifedigbo, K Nakahira, T Dolinay… - Autophagy, 2011 - Taylor & Francis
Macroautophagy is a highly conserved catabolic process that is crucial for organ
homeostasis in mammals. However, methods to directly measure macroautophagic activity …

Photothermal exposure of polydopamine-coated branched Au–Ag nanoparticles induces cell cycle arrest, apoptosis, and autophagy in human bladder cancer cells

X Zhao, T Qi, C Kong, M Hao, Y Wang, J Li… - International journal …, 2018 - Taylor & Francis
Purpose Polydopamine-coated branched Au–Ag nanoparticles (Au–Ag@ PDA NPs) exhibit
good structural stability, biocompatibility, and photothermal performance, along with …