Autophagosome biogenesis is a dynamic membrane event, which is executed by the
sequential function of autophagy-related (ATG) proteins. Upon autophagy induction, a cup …

Coat protein complex I (COPI) and COPII are required for bidirectional membrane trafficking
between the endoplasmic reticulum (ER) and the Golgi. While these core coat machineries …

The Drosophila genome contains> 13000 protein-coding genes, the majority of which
remain poorly investigated. Important reasons include the lack of antibodies or reporter …

To dissect secretory traffic, we developed the retention using selective hooks (RUSH)
system. RUSH is a two-state assay based on the reversible interaction of a hook protein …

Few components of the mitochondrial fission machinery are known, even though
mitochondrial fission is a complex process of vital importance for cell growth and survival …

RNA interference (RNAi) has become a powerful technique for reverse genetics and drug
discovery, and in both of these areas large-scale high-throughput RNAi screens are …

We demonstrate the first use of smartphone spectrophotometry for readout of fluorescence-
based biological assays. We evaluated the smartphone fluorimeter in the context of a …

Recent studies by our group and others demonstrated a required and conserved role of Stim
in store-operated Ca2+ influx and Ca2+ release-activated Ca2+ (CRAC) channel activity. By …

Eukaryotic cells store neutral lipids in cytoplasmic lipid droplets, enclosed in a monolayer of
phospholipids and associated proteins,. These dynamic organelles serve as the principal …

Lipid droplets (LDs) are ubiquitous organelles that store neutral lipids, such as
triacylglycerol (TG), as reservoirs of metabolic energy and membrane precursors. The …