Advances and challenges in small‐molecule DNA aptamer isolation, characterization, and sensor development
Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind
to specific molecules with high affinity, and offer a number of advantages relative to …
to specific molecules with high affinity, and offer a number of advantages relative to …
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment
Aptamers are short single-stranded DNA or RNA oligonucleotides isolated from randomized
libraries, presenting high affinity and specificity toward targets with similar to antibodies …
libraries, presenting high affinity and specificity toward targets with similar to antibodies …
Critical evaluation of aptamer binding for biosensor designs
Over the last three decades, numerous aptamer-based biosensors have been reported. The
basis of these sensors is the selective binding of target analytes by aptamers. In the last few …
basis of these sensors is the selective binding of target analytes by aptamers. In the last few …
Label-free and dye-free fluorescent sensing of tetracyclines using a capture-selected DNA aptamer
Y Zhao, S Ong, Y Chen, PJ Jimmy Huang… - Analytical …, 2022 - ACS Publications
Tetracyclines are a group of important antibiotics with a common four-ring scaffold. While
most tetracyclines are currently used only in animals, their leaching into the environment …
most tetracyclines are currently used only in animals, their leaching into the environment …
Updates on aptamer research
MH Ali, ME Elsherbiny, M Emara - International journal of molecular …, 2019 - mdpi.com
For many years, different probing techniques have mainly relied on antibodies for molecular
recognition. However, with the discovery of aptamers, this has changed. The science …
recognition. However, with the discovery of aptamers, this has changed. The science …
Innovative engineering and sensing strategies for aptamer-based small-molecule detection
Aptamers are nucleic acid-based affinity reagents that have gained widespread attention as
biorecognition elements for the detection of targets such as ions, small molecules, and …
biorecognition elements for the detection of targets such as ions, small molecules, and …
Perspective on the future role of aptamers in analytical chemistry
It has been almost 30 years since the invention of Systematic Evolution of Ligands by
Exponential Enrichment (SELEX) methodology and the description of the first aptamers. In …
Exponential Enrichment (SELEX) methodology and the description of the first aptamers. In …
Selection of aptamers for sensing caffeine and discrimination of its three single demethylated analogues
With the growing consumption of caffeine-containing beverages, detection of caffeine has
become an important biomedical, bioanalytical, and environmental topic. We herein isolated …
become an important biomedical, bioanalytical, and environmental topic. We herein isolated …
A DNA aptamer for theophylline with ultrahigh selectivity reminiscent of the classic RNA aptamer
Since the report of the RNA aptamer for theophylline, theophylline has become a key
molecule in chemical biology for designing RNA switches and riboswitches. In addition …
molecule in chemical biology for designing RNA switches and riboswitches. In addition …
Using exonucleases for aptamer characterization, engineering, and sensing
O Alkhamis, J Canoura, PT Ly… - Accounts of Chemical …, 2023 - ACS Publications
Conspectus Aptamers are short, single-stranded nucleic acids that have been selected from
random libraries to bind specific molecules with high affinity via an in vitro method termed …
random libraries to bind specific molecules with high affinity via an in vitro method termed …