Advances and challenges in small‐molecule DNA aptamer isolation, characterization, and sensor development

H Yu, O Alkhamis, J Canoura, Y Liu… - Angewandte Chemie …, 2021 - Wiley Online Library
Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind
to specific molecules with high affinity, and offer a number of advantages relative to …

Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment

S Qi, N Duan, IM Khan, X Dong, Y Zhang, S Wu… - Biotechnology …, 2022 - Elsevier
Aptamers are short single-stranded DNA or RNA oligonucleotides isolated from randomized
libraries, presenting high affinity and specificity toward targets with similar to antibodies …

Critical evaluation of aptamer binding for biosensor designs

Y Zhao, K Yavari, J Liu - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
Over the last three decades, numerous aptamer-based biosensors have been reported. The
basis of these sensors is the selective binding of target analytes by aptamers. In the last few …

Label-free and dye-free fluorescent sensing of tetracyclines using a capture-selected DNA aptamer

Y Zhao, S Ong, Y Chen, PJ Jimmy Huang… - Analytical …, 2022 - ACS Publications
Tetracyclines are a group of important antibiotics with a common four-ring scaffold. While
most tetracyclines are currently used only in animals, their leaching into the environment …

Updates on aptamer research

MH Ali, ME Elsherbiny, M Emara - International journal of molecular …, 2019 - mdpi.com
For many years, different probing techniques have mainly relied on antibodies for molecular
recognition. However, with the discovery of aptamers, this has changed. The science …

Innovative engineering and sensing strategies for aptamer-based small-molecule detection

O Alkhamis, J Canoura, H Yu, Y Liu, Y Xiao - TrAC Trends in Analytical …, 2019 - Elsevier
Aptamers are nucleic acid-based affinity reagents that have gained widespread attention as
biorecognition elements for the detection of targets such as ions, small molecules, and …

Perspective on the future role of aptamers in analytical chemistry

Y Wu, I Belmonte, KS Sykes, Y Xiao… - Analytical …, 2019 - ACS Publications
It has been almost 30 years since the invention of Systematic Evolution of Ligands by
Exponential Enrichment (SELEX) methodology and the description of the first aptamers. In …

Selection of aptamers for sensing caffeine and discrimination of its three single demethylated analogues

PJJ Huang, J Liu - Analytical Chemistry, 2022 - ACS Publications
With the growing consumption of caffeine-containing beverages, detection of caffeine has
become an important biomedical, bioanalytical, and environmental topic. We herein isolated …

A DNA aptamer for theophylline with ultrahigh selectivity reminiscent of the classic RNA aptamer

PJJ Huang, J Liu - ACS Chemical Biology, 2022 - ACS Publications
Since the report of the RNA aptamer for theophylline, theophylline has become a key
molecule in chemical biology for designing RNA switches and riboswitches. In addition …

Using exonucleases for aptamer characterization, engineering, and sensing

O Alkhamis, J Canoura, PT Ly… - Accounts of Chemical …, 2023 - ACS Publications
Conspectus Aptamers are short, single-stranded nucleic acids that have been selected from
random libraries to bind specific molecules with high affinity via an in vitro method termed …