Towards organoid culture without Matrigel

MT Kozlowski, CJ Crook, HT Ku - Communications biology, 2021 - nature.com
Organoids—cellular aggregates derived from stem or progenitor cells that recapitulate organ
function in miniature—are of growing interest in developmental biology and medicine …

Decellularized extracellular matrix: New promising and challenging biomaterials for regenerative medicine

M Brown, J Li, C Moraes, M Tabrizian, NYK Li-Jessen - Biomaterials, 2022 - Elsevier
Extracellular matrix is rich in biomolecules including structural proteins,
glycosaminoglycans, and small molecules that are important for the maintenance and repair …

Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids

S Kim, S Min, YS Choi, SH Jo, JH Jung, K Han… - Nature …, 2022 - nature.com
Matrigel, a mouse tumor extracellular matrix protein mixture, is an indispensable component
of most organoid tissue culture. However, it has limited the utility of organoids for drug …

Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

AN Cho, Y Jin, Y An, J Kim, YS Choi, JS Lee… - Nature …, 2021 - nature.com
Brain organoids derived from human pluripotent stem cells provide a highly valuable in vitro
model to recapitulate human brain development and neurological diseases. However, the …

Decellularized extracellular matrix-based bioinks for engineering tissue-and organ-specific microenvironments

BS Kim, S Das, J Jang, DW Cho - Chemical reviews, 2020 - ACS Publications
Biomaterials-based biofabrication methods have gained much attention in recent years.
Among them, 3D cell printing is a pioneering technology to facilitate the recapitulation of …

Extracellular matrix-based materials for regenerative medicine

GS Hussey, JL Dziki, SF Badylak - Nature Reviews Materials, 2018 - nature.com
In tissue engineering and regenerative medicine, a biomaterial provides mechanical support
and biochemical signals to encourage cell attachment and modulate cell behaviour …

Extracellular matrix hydrogels from decellularized tissues: Structure and function

LT Saldin, MC Cramer, SS Velankar, LJ White… - Acta biomaterialia, 2017 - Elsevier
Extracellular matrix (ECM) bioscaffolds prepared from decellularized tissues have been
used to facilitate constructive and functional tissue remodeling in a variety of clinical …

Rapid 3D bioprinting of decellularized extracellular matrix with regionally varied mechanical properties and biomimetic microarchitecture

X Ma, C Yu, P Wang, W Xu, X Wan, CSE Lai, J Liu… - Biomaterials, 2018 - Elsevier
Hepatocellular carcinoma (HCC), as the fifth most common malignant cancer, develops and
progresses mostly in a cirrhotic liver where stiff nodules are separated by fibrous bands …

Extracellular matrix hydrogel therapies: In vivo applications and development

MT Spang, KL Christman - Acta biomaterialia, 2018 - Elsevier
Decellularized extracellular matrix (ECM) has been widely used for tissue engineering
applications and is becoming increasingly versatile as it can take many forms, including …

Designing decellularized extracellular matrix‐based bioinks for 3D bioprinting

A Abaci, M Guvendiren - Advanced healthcare materials, 2020 - Wiley Online Library
Abstract 3D bioprinting is an emerging technology to fabricate tissues and organs by
precisely positioning cells into 3D structures using printable cell‐laden formulations known …