Microbial tolerance engineering toward biochemical production: from lignocellulose to products

H Ling, W Teo, B Chen, SSJ Leong… - Current opinion in …, 2014 - Elsevier
Highlights•Microbial metabolic engineering enables biochemical production at high
efficiency.•Bioprocessing of lignocellulose to products generates various toxic …

Engineering metabolic pathways in Escherichia coli for constructing a “microbial chassis” for biochemical production

T Matsumoto, T Tanaka, A Kondo - Bioresource technology, 2017 - Elsevier
The present work reviews literature describing the re-design of the metabolic pathways of a
microbial host using sophisticated genetic tools, yielding strains for producing value-added …

Enhancing Tolerance to Short-Chain Alcohols by Engineering the Escherichia coli AcrB Efflux Pump to Secrete the Non-native Substrate n-Butanol

MA Fisher, S Boyarskiy, MR Yamada, N Kong… - ACS synthetic …, 2014 - ACS Publications
The microbial conversion of sugars to fuels is a promising technology, but the byproducts of
biomass pretreatment processes and the fuels themselves are often toxic at industrially …

Engineering a short, aldolase-based pathway for (R)-1, 3-butanediol production in Escherichia coli

K Nemr, JEN Müller, JC Joo, P Gawand… - Metabolic …, 2018 - Elsevier
Microbial processes can produce a wide range of compounds; however, producing complex
and long chain hydrocarbons remains a challenge. Aldol condensation offers a direct route …

Robust microorganisms for biofuel and chemical production from municipal solid waste

A Dornau, JF Robson, GH Thomas… - Microbial cell …, 2020 - Springer
Background Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced
annually by 2050, however, current approaches to MSW management predominantly …

Fermentation of lactose to ethanol in cheese whey permeate and concentrated permeate by engineered Escherichia coli

L Pasotti, S Zucca, M Casanova, G Micoli… - BMC …, 2017 - Springer
Background Whey permeate is a lactose-rich effluent remaining after protein extraction from
milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation …

Genome engineering in cyanobacteria: where we are and where we need to go

CJ Ramey, A Barón-Sola, HR Aucoin… - ACS synthetic …, 2015 - ACS Publications
Genome engineering of cyanobacteria is a promising area of development in order to
produce fuels, feedstocks, and value-added chemicals in a sustainable way. Unfortunately …

OptEnvelope: A target point guided method for growth-coupled production using knockouts

E Motamedian, K Berzins, R Muiznieks, E Stalidzans - Plos one, 2023 - journals.plos.org
Finding the best knockout strategy for coupling biomass growth and production of a target
metabolite using a mathematic model of metabolism is a challenge in biotechnology. In this …

Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol

L Tian, SJ Perot, D Stevenson, T Jacobson… - Biotechnology for …, 2017 - Springer
Background Clostridium thermocellum is a promising microorganism for conversion of
cellulosic biomass to biofuel, without added enzymes; however, the low ethanol titer …

[HTML][HTML] The LASER database: Formalizing design rules for metabolic engineering

JD Winkler, AL Halweg-Edwards, RT Gill - Metabolic Engineering …, 2015 - Elsevier
The ability of metabolic engineers to conceptualize, implement, and evaluate strain designs
has dramatically increased in the last decade. Unlike other engineering fields, no …