Quantitative genome-wide enhancer activity maps for five Drosophila species show functional enhancer conservation and turnover during cis-regulatory evolution
CD Arnold, D Gerlach, D Spies, JA Matts… - Nature …, 2014 - nature.com
Phenotypic differences between closely related species are thought to arise primarily from
changes in gene expression due to mutations in cis-regulatory sequences (enhancers) …
changes in gene expression due to mutations in cis-regulatory sequences (enhancers) …
Conserved piRNA expression from a distinct set of piRNA cluster loci in eutherian mammals
G Chirn, R Rahman, YA Sytnikova, JA Matts… - PLoS …, 2015 - journals.plos.org
The Piwi pathway is deeply conserved amongst animals because one of its essential
functions is to repress transposons. However, many Piwi-interacting RNAs (piRNAs) do not …
functions is to repress transposons. However, many Piwi-interacting RNAs (piRNAs) do not …
Identification of extracellular miRNA in archived serum samples by next-generation sequencing from RNA extracted using multiple methods
Abstract miRNAs act as important regulators of gene expression by promoting mRNA
degradation or by attenuating protein translation. Since miRNAs are stably expressed in …
degradation or by attenuating protein translation. Since miRNAs are stably expressed in …
Transposable element dynamics and PIWI regulation impacts lncRNA and gene expression diversity in Drosophila ovarian cell cultures
YA Sytnikova, R Rahman, G Chirn, JP Clark… - Genome …, 2014 - genome.cshlp.org
Piwi proteins and Piwi-interacting RNAs (piRNAs) repress transposable elements (TEs) from
mobilizing in gonadal cells. To determine the spectrum of piRNA-regulated targets that may …
mobilizing in gonadal cells. To determine the spectrum of piRNA-regulated targets that may …
Boronate affinity electrophoresis for the purification and analysis of cofactor-modified RNAs
G Nübel, FA Sorgenfrei, A Jäschke - Methods, 2017 - Elsevier
RNA modifications are widely distributed in Nature, and their thorough analysis helps
answering fundamental biological questions. Nowadays, mass spectrometry or deep …
answering fundamental biological questions. Nowadays, mass spectrometry or deep …
RNase H-based analysis of synthetic mRNA 5′ cap incorporation
SH Chan, JM Whipple, N Dai, TM Kelley, K Withers… - Rna, 2022 - rnajournal.cshlp.org
Advances in mRNA synthesis and lipid nanoparticles technologies have helped make
mRNA therapeutics and vaccines a reality. The 5′ cap structure is a crucial modification …
mRNA therapeutics and vaccines a reality. The 5′ cap structure is a crucial modification …
Expression of microRNAs during female inflorescence development in African oil palm (Elaeis guineensis Jacq.)
H Ho, R Gudimella, M Ong-Abdullah… - Tree Genetics & …, 2017 - Springer
In African oil palm, the formation of fruit relies on the successful progression of a 2-to 3-year
phase of development of inflorescences, in particular the female inflorescence. In this study …
phase of development of inflorescences, in particular the female inflorescence. In this study …
Improved boronate affinity electrophoresis by optimization of the running buffer for a single-step separation of piRNA from mouse testis total RNA
Y Sato, D Iwasawa, KP Hui, R Nakagomi… - Analytical …, 2018 - jstage.jst.go.jp
Here we examined optimization of the running buffer in boronate affinity electrophoresis for
improved separation of PIWI-interacting RNA (piRNA) with 2′-O-methylated ribose in 3 …
improved separation of PIWI-interacting RNA (piRNA) with 2′-O-methylated ribose in 3 …
[HTML][HTML] PBOX-sRNA-seq uncovers novel features of miRNA modification and identifies selected 5′-tRNA fragments bearing 2′-O-modification
S Chen, Y Cai, H Yang, B Zhang, N Li, G Ren - Nucleic Acids Research - academic.oup.com
The concomitant cloning of RNA degradation products is a major concern in standard small
RNA-sequencing practices. This not only complicates the characterization of bona fide …
RNA-sequencing practices. This not only complicates the characterization of bona fide …
Boronic acid assisted assembly and activity control of split nucleic acid enzymes
A Lelievre - 2022 - epub.ub.uni-greifswald.de
The hairpin ribozyme is a small Mg2+-dependent catalytic RNA molecule able to catalyze
the trans-cleavage of an RNA substrate via a reversible trans-esterification mechanism. In …
the trans-cleavage of an RNA substrate via a reversible trans-esterification mechanism. In …