Eukaryotic and prokaryotic organisms possess huge numbers of uncharacterized enzymes.
Selective inhibitors offer powerful probes for assigning functions to enzymes in native …

The caseinolytic protease proteolytic subunit (ClpP) is a serine protease playing an
important role in proteostasis of eukaryotic organelles and prokaryotic cells. Alteration of …

Orlistat, or tetrahydrolipstatin (THL), is an FDA-approved antiobesity drug with potential
antitumor activities. Cellular off-targets and potential side effects of Orlistat in cancer …

Target practice: The target of the antibiotic cyclomarin A was identified in Mycobacterium.
Cyclomarin A (see structure) binds the regulatory subunit of the Clp protease complex with …

Intracellular proteolysis carried out by energy-dependent proteases is one of the most
conserved biological processes. In all cells proteolysis maintains and shapes the cellular …

Activity‐based protein profiling (ABPP) has matured into a standard method for the fast,
sensitive, and selective identification of enzyme activity and inhibitors in proteomes. By …

The caseinolytic protease (ClpP) is part of a highly conserved proteolytic complex whose
disruption can lead to antibacterial activity but for which few specific inhibitors have been …

The Clp protease complex degrades a multitude of substrates, which are engaged by a
AAA+ chaperone such as ClpX and subsequently digested by the dynamic, barrel-shaped …

Fluorescence microscopy is an essential tool for the exploration of cell growth, division,
transcription and translation in eukaryotes and prokaryotes alike. Despite the rapid …

Covering: up to 2017 The bacterial Clp protease is a highly conserved and structurally
versatile machine. It has gained a lot of recognition during the last decade as a novel …