Substrate channelling as an approach to cascade reactions
Millions of years of evolution have produced biological systems capable of efficient one-pot
multi-step catalysis. The underlying mechanisms that facilitate these reaction processes are …
multi-step catalysis. The underlying mechanisms that facilitate these reaction processes are …
Biocatalytic cascades operating on macromolecular scaffolds and in confined environments
Biocatalytic cascades guide complex, efficient and selective intracellular transformations.
These unique features originate from the spatial organization of the biocatalysts in confined …
These unique features originate from the spatial organization of the biocatalysts in confined …
Self assembling nanoparticle enzyme clusters provide access to substrate channeling in multienzymatic cascades
Access to efficient enzymatic channeling is desired for improving all manner of designer
biocatalysis. We demonstrate that enzymes constituting a multistep cascade can self …
biocatalysis. We demonstrate that enzymes constituting a multistep cascade can self …
Nanocaged enzymes with enhanced catalytic activity and increased stability against protease digestion
Cells routinely compartmentalize enzymes for enhanced efficiency of their metabolic
pathways. Here we report a general approach to construct DNA nanocaged enzymes for …
pathways. Here we report a general approach to construct DNA nanocaged enzymes for …
Enhanced activity of immobilized or chemically modified enzymes
Re-engineering enzymes with high activities in the given environments different from the
physiological one has been constantly pursued for application of enzymatic catalysis in …
physiological one has been constantly pursued for application of enzymatic catalysis in …
[HTML][HTML] Proximity does not contribute to activity enhancement in the glucose oxidase–horseradish peroxidase cascade
A proximity effect has been invoked to explain the enhanced activity of enzyme cascades on
DNA scaffolds. Using the cascade reaction carried out by glucose oxidase and horseradish …
DNA scaffolds. Using the cascade reaction carried out by glucose oxidase and horseradish …
Reconfigurable DNA origami nanocapsule for pH-controlled encapsulation and display of cargo
DNA nanotechnology provides a toolbox for creating custom and precise nanostructures
with nanometer-level accuracy. These nano-objects are often static by nature and serve as …
with nanometer-level accuracy. These nano-objects are often static by nature and serve as …
From protein film electrochemistry to nanoconfined enzyme cascades and the electrochemical leaf
Protein film electrochemistry (PFE) has given unrivalled insight into the properties of redox
proteins and many electron-transferring enzymes, allowing investigations of otherwise ill …
proteins and many electron-transferring enzymes, allowing investigations of otherwise ill …
Artificial Multienzyme Scaffolds: Pursuing in Vitro Substrate Channeling with an Overview of Current Progress
Artificial multienzyme scaffolds are being developed for in vitro cascaded biocatalytic activity
and, in particular, accessing substrate channeling. This review covers progress in this field …
and, in particular, accessing substrate channeling. This review covers progress in this field …
Engineered repeat proteins as scaffolds to assemble multi-enzyme systems for efficient cell-free biosynthesis
A Ledesma-Fernandez, S Velasco-Lozano… - Nature …, 2023 - nature.com
Multi-enzymatic cascades with enzymes arranged in close-proximity through a protein
scaffold can trigger a substrate channeling effect, allowing for efficient cofactor reuse with …
scaffold can trigger a substrate channeling effect, allowing for efficient cofactor reuse with …