Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle
Simple Summary A high incidence of early pregnancy failures exists in beef and dairy cows.
This review describes various problems which occur during early gestation that contribute to …
This review describes various problems which occur during early gestation that contribute to …
Ablation of OCT4 function in cattle embryos by double electroporation of CRISPR-Cas for DNA and RNA targeting (CRISPR-DART)
CRISPR-Cas ribonucleoproteins (RNPs) are important tools for gene editing in
preimplantation embryos. However, the inefficient production of biallelic deletions in cattle …
preimplantation embryos. However, the inefficient production of biallelic deletions in cattle …
The impact of cumulus cell viability and pre-culture with the healthy cell mass on brilliant cresyl blue (BCB) staining assessment and meiotic competence of suboptimal …
WTL Fonseka, SQ Do, PN Van, HT Nguyen, T Wakai… - Theriogenology, 2024 - Elsevier
Objectives of the present study were to investigate the characteristics including glucose-6-
phosphate dehydrogenase activity, as determined by Brilliant Cresyl Blue (BCB) staining, of …
phosphate dehydrogenase activity, as determined by Brilliant Cresyl Blue (BCB) staining, of …
[HTML][HTML] Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes
FH Biase, G Schettini - STAR protocols, 2024 - Elsevier
The use of CRISPR-Cas9 ribonucleoproteins has revolutionized manipulation of genomes.
Here, we present a protocol for the electroporation of CRISPR-Cas for DNA and RNA …
Here, we present a protocol for the electroporation of CRISPR-Cas for DNA and RNA …
Identification of novel genes in cattle (Bos taurus) and biological insights into their function in embryo development
GP Schettini, M Morozyuk, FH Biase - bioRxiv, 2024 - biorxiv.org
Appropriate regulation of genes expressed in oocytes and embryos is essential for
acquisition of developmental competence in mammals. Here, we hypothesized that several …
acquisition of developmental competence in mammals. Here, we hypothesized that several …
Exploring methods to understand bovine embryo competency in vitro
JL Nix - 2023 - vtechworks.lib.vt.edu
The development of a preimplantation embryo is a stepwise process consisting of
morphological, biochemical, and genomic changes. Much remains unknown about the …
morphological, biochemical, and genomic changes. Much remains unknown about the …