Challenges in implementing plant shoot tip cryopreservation technologies

JC Bettoni, R Bonnart, GM Volk - Plant Cell, Tissue and Organ Culture …, 2021 - Springer
Cryopreservation is a safe approach for the long-term preservation of plant genetic
resources that complements traditional field and in vitro germplasm collections. There are …

[HTML][HTML] Plant protection from virus: a review of different approaches

I Anikina, A Kamarova, K Issayeva… - Frontiers in Plant …, 2023 - frontiersin.org
This review analyzes methods for controlling plant viral infection. The high harmfulness of
viral diseases and the peculiarities of viral pathogenesis impose special requirements …

[HTML][HTML] Eradication of Potato Virus S, Potato Virus A, and Potato Virus M From Infected in vitro-Grown Potato Shoots Using in vitro Therapies

JC Bettoni, L Mathew, R Pathirana, C Wiedow… - Frontiers in Plant …, 2022 - frontiersin.org
Certain viruses dramatically affect yield and quality of potatoes and have proved difficult to
eradicate with current approaches. Here, we describe a reliable and efficient virus …

[HTML][HTML] Grapevine shoot tip cryopreservation and cryotherapy: Secure storage of disease-free plants

JC Bettoni, Z Marković, W Bi, GM Volk, T Matsumoto… - Plants, 2021 - mdpi.com
Grapevine (Vitis spp.) is one of the most economically important temperate fruit crops.
Grapevine breeding programs require access to high-quality Vitis cultivars and wild species …

[HTML][HTML] Thermotherapy followed by shoot tip cryotherapy eradicates latent viruses and apple hammerhead viroid from in vitro apple rootstocks

JC Bettoni, G Fazio, L Carvalho Costa… - Plants, 2022 - mdpi.com
Virus and viroid-free apple rootstocks are necessary for large-scale nursery propagation of
apple (Malus domestica) trees. Apple stem grooving virus (ASGV) and Apple chlorotic leaf …

Shoot tip cryotherapy for plant pathogen eradication

MR Wang, WL Bi, JC Bettoni, D Zhang… - Plant …, 2022 - Wiley Online Library
Diseases caused by plant pathogens such as viruses, viroids and phytoplasmas cause huge
economic losses of agricultural production and limit the safe movement of plant materials …

Application of biotechniques for in vitro virus and viroid elimination in pome fruit crops

JC Bettoni, MR Wang, JW Li, X Fan, G Fazio… - …, 2024 - Am Phytopath Society
Sustainable production of pome fruit crops is dependent upon having virus-free planting
materials. The production and distribution of plants derived from virus-and viroid-negative …

Droplet-vitrification cryotherapy and thermotherapy as efficient tools for the eradication of apple chlorotic leaf spot virus and apple stem grooving virus from virus …

S Farhadi-Tooli, A Ghanbari, MJ Kermani… - European Journal of …, 2022 - Springer
Viral diseases affect quince plant productivity and fruit quality. This study evaluated the
effectiveness of droplet-vitrification cryotherapy and thermotherapy methods in the …

[HTML][HTML] Cryopreservation of 12 Vitis species using apical shoot tips derived from plants grown in vitro

JC Bettoni, AA Kretzschmar, R Bonnart, A Shepherd… - …, 2019 - journals.ashs.org
The availability of and easy access to diverse Vitis species are prerequisites for advances in
breeding programs. Plant genebanks usually maintain collections of Vitis taxa as field …

[HTML][HTML] Phytotoxicity and other adverse effects on the in vitro shoot cultures caused by virus elimination treatments: Reasons and solutions

K Magyar-Tábori, N Mendler-Drienyovszki, A Hanász… - Plants, 2021 - mdpi.com
In general, in vitro virus elimination is based on the culture of isolated meristem, and in
addition thermotherapy, chemotherapy, electrotherapy, and cryotherapy can also be …