Cell-free gene expression

D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

A user's guide to golden gate cloning methods and standards

JE Bird, J Marles-Wright, A Giachino - ACS Synthetic Biology, 2022 - ACS Publications
The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

Scale‐up economics for cultured meat

D Humbird - Biotechnology and Bioengineering, 2021 - Wiley Online Library
This analysis examines the potential of “cultured meat” products made from edible animal
cell culture to measurably displace the global consumption of conventional meat …

Strategies for improving antimicrobial peptide production

S Deo, KL Turton, T Kainth, A Kumar, HJ Wieden - Biotechnology advances, 2022 - Elsevier
Antimicrobial peptides (AMPs) found in a wide range of animal, insect, and plant species are
host defense peptides forming an integral part of their innate immunity. Although the exact …

A glucose meter interface for point-of-care gene circuit-based diagnostics

E Amalfitano, M Karlikow, M Norouzi, K Jaenes… - Nature …, 2021 - nature.com
Recent advances in cell-free synthetic biology have given rise to gene circuit-based sensors
with the potential to provide decentralized and low-cost molecular diagnostics. However, it …

The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform

D Garenne, S Thompson, A Brisson… - Synthetic …, 2021 - academic.oup.com
The new generation of cell-free gene expression systems enables the prototyping and
engineering of biological systems in vitro over a remarkable scope of applications and …

Advances in cell‐free biosensors: principle, mechanism, and applications

L Zhang, W Guo, Y Lu - Biotechnology Journal, 2020 - Wiley Online Library
Synthetic biology has promoted the development of biosensors as tools for detecting trace
substances. In the past, biosensors based on synthetic biology have been designed on …

Bottom-up construction of complex biomolecular systems with cell-free synthetic biology

N Laohakunakorn, L Grasemann… - … in bioengineering and …, 2020 - frontiersin.org
Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …

The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology

R Biedendieck, T Knuuti, SJ Moore, D Jahn - Applied microbiology and …, 2021 - Springer
Over 30 years, the Gram-positive bacterium Priestia megaterium (previously known as
Bacillus megaterium) was systematically developed for biotechnological applications …

Advances in riboswitch‐based biosensor as food samples detection tool

X Dong, S Qi, IM Khan, Y Sun… - … Reviews in Food …, 2023 - Wiley Online Library
Food safety has always been a hot issue of social concern, and biosensing has been widely
used in the field of food safety detection. Compared with traditional aptamer‐based …