Dual-affinity graphene sheets for high-resolution cryo-electron microscopy

H Cheng, L Zheng, N Liu, C Huang, J Xu… - Journal of the …, 2023 - ACS Publications
With the development of cryo-electron microscopy (cryo-EM), high-resolution structures of
macromolecules can be reconstructed by the single particle method efficiently. However …

Mass-selective and ice-free electron cryomicroscopy protein sample preparation via native electrospray ion-beam deposition

TK Esser, J Böhning, P Fremdling, MT Agasid… - PNAS …, 2022 - academic.oup.com
Despite tremendous advances in sample preparation and classification algorithms for
electron cryomicroscopy (cryo-EM) and single-particle analysis (SPA), sample heterogeneity …

Affinity capture of p97 with small-molecule ligand bait reveals a 3.6 Å double-hexamer cryoelectron microscopy structure

MR Hoq, FS Vago, K Li, M Kovaliov, RJ Nicholas… - ACS …, 2021 - ACS Publications
Recent progress in the development of affinity grids for cryoelectron microscopy (cryo-EM)
typically employs genetic engineering of the protein sample such as histidine or Spy …

Atomically thin bilayer janus membranes for cryo-electron microscopy

L Zheng, N Liu, Y Liu, N Li, J Zhang, C Wang, W Zhu… - ACS …, 2021 - ACS Publications
Cryo-electron microscopy (cryo-EM) has emerged as a vital tool to reveal the native structure
of beam-sensitive biomolecules and materials. Yet high-resolution cryo-EM analysis is still …

Tuning the Properties of Poly (ethylene glycol) Films and Membranes by the Molecular Weight of the Precursors

Z Zhao, S Das, M Zharnikov - ACS Applied Polymer Materials, 2021 - ACS Publications
We show that ultrathin poly (ethylene glycol)(PEG) films and freestanding membranes can
be readily prepared by thermally activated cross-linking of amine/epoxy-decorated STAR …

An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM

Q Zhao, X Hong, Y Wang, S Zhang, Z Ding… - Communications …, 2024 - nature.com
In cryo-electron microscopy (cryo-EM), sample preparation poses a critical bottleneck,
particularly for rare or fragile macromolecular assemblies and those suffering from …

Preparation and characterization of highly conductive and biorepulsive polypyrrole/polyglycerol surface films

S Balser, J Bernd, L Fritsche… - ACS Applied Polymer …, 2022 - ACS Publications
Highly conductive and biorepulsive polymer films can be deposited onto gold by
electrochemical polymerization of a polyglycerol-modified pyrrole derivative in aqueous …

Synergistic Functionalization of Graphene Oxide: Electrochemical Devices and Ritter Catalysis

MS Ahmad, Y Nishina, Y Inomata… - The Journal of …, 2024 - ACS Publications
Graphene oxide (GO) is a versatile platform with unique properties that find broad
applications in electronics and catalysis. The double covalent functionalization of GO under …

Development of immobilized antibody-based affinity grid strategy and application in on-grid purification of target proteins for high-resolution cryo-EM

Q Zhao, X Hong, Y Wang, S Zhang, Z Ding, X Meng… - bioRxiv, 2024 - biorxiv.org
In cryo-electron microscopy (cryo-EM), sample preparation, especially for rare or fragile
macromolecular assemblies and those suffering from air-water interface denaturation and …

Elastic properties of poly (ethylene glycol) nanomembranes and respective implications

Z Zhao, M Zharnikov - Membranes, 2022 - mdpi.com
Free-standing poly (ethylene glycol)(PEG) membranes were prepared from amine-and
epoxy-terminated four-arm STAR-PEG precursors in a thickness range of 40–320 nm. The …