The epigenetic modifications of histones are versatile marks that are intimately connected to
development and disease pathogenesis including human cancers. In this review, we will …

Cell-type-and condition-specific profiles of gene expression require coordination between
protein-coding gene promoters and cis-regulatory sequences called enhancers. Enhancers …

Regulatable CAR platforms could circumvent toxicities associated with CAR-T therapy, but
existing systems have shortcomings including leakiness and attenuated activity. Here, we …

Protein knockdown using the auxin-inducible degron (AID) technology is useful to study
protein function in living cells because it induces rapid depletion, which makes it possible to …

Dissection of complex biological systems requires target-specific control of the function or
abundance of proteins. Genetic perturbations are limited by off-target effects …

Cell-based therapies are emerging as effective agents against cancer and other diseases.
As autonomous “living drugs,” these therapies lack precise control. Chimeric antigen …

Studying the role of essential proteins is dependent upon a method for rapid inactivation, in
order to study the immediate phenotypic consequences. Auxin-inducible degron (AID) …

Controlled perturbation of protein activity is essential to study protein function in cells and
living organisms. Small molecules that hijack the cellular protein ubiquitination machinery to …

Chemical biology strategies for directly perturbing protein homeostasis including the
degradation tag (dTAG) system provide temporal advantages over genetic approaches and …

While Mediator plays a key role in eukaryotic transcription, little is known about its
mechanism of action. This study combines CRISPR-Cas9 genetic screens, degron assays …