Versatility of reverse micelles: From biomimetic models to nano (bio) sensor design

ML Arsene, I Răut, M Călin, ML Jecu, M Doni… - Processes, 2021 - mdpi.com
This paper presents an overview of the principal structural and dynamics characteristics of
reverse micelles (RMs) in order to highlight their structural flexibility and versatility, along …

Cell-size space effects on phase separation of binary polymer blends

M Yanagisawa - Biophysical Reviews, 2022 - Springer
Within living cells, a diverse array of biomolecules is present at high concentrations. To
better understand how molecular behavior differs under such conditions (collectively …

Cell-free protein synthesis inside giant unilamellar vesicles analyzed by flow cytometry

K Nishimura, T Matsuura, K Nishimura, T Sunami… - Langmuir, 2012 - ACS Publications
Lipid vesicles have been used as model cell systems, in which an in-vitro transcription–
translation system (IVTT) is encapsulated to carry out intravesicular protein synthesis …

Oriented reconstitution of a membrane protein in a giant unilamellar vesicle: experimental verification with the potassium channel KcsA

M Yanagisawa, M Iwamoto, A Kato… - Journal of the …, 2011 - ACS Publications
We report a method for the successful reconstitution of the KcsA potassium channel with
either an outside-out or inside-out orientation in giant unilamellar vesicles, using the droplet …

Size control of giant unilamellar vesicles prepared from inverted emulsion droplets

K Nishimura, H Suzuki, T Toyota, T Yomo - Journal of colloid and interface …, 2012 - Elsevier
The production of giant lipid vesicles with controlled size and structure will be an important
technology in the design of quantitative biological assays in cell-mimetic …

Cell-sized confinement in microspheres accelerates the reaction of gene expression

A Kato, M Yanagisawa, YT Sato, K Fujiwara… - Scientific reports, 2012 - nature.com
Cell-sized water-in-oil droplet covered by a lipid layer was used to understand how lipid
membranes affect biochemical systems in living cells. Here, we report a remarkable …

Reentrant DNA shells tune polyphosphate condensate size

R Chawla, JKA Tom, T Boyd, NH Tu, T Bai… - Nature …, 2024 - nature.com
The inorganic biopolymer polyphosphate (polyP) occurs in all domains of life and affects
myriad cellular processes. A longstanding observation is polyP's frequent proximity to …

Semiflexible polymers in spherical confinement: Bipolar orientational order versus tennis ball states

A Nikoubashman, DA Vega, K Binder, A Milchev - Physical review letters, 2017 - APS
Densely packed semiflexible polymers with contour length L confined in spheres with radius
R of the same order as L cannot exhibit uniform nematic order. Depending on the chain …

Cell-sized liposomes and droplets: Real-world modeling of living cells

T Hamada, K Yoshikawa - Materials, 2012 - mdpi.com
Recent developments in studies concerning cell-sized vesicles, such as liposomes with a
lipid bilayer and water-in-oil droplets covered by a lipid monolayer, aim to realize the real …

Specific spatial localization of actin and DNA in a water/water microdroplet: self‐emergence of a cell‐like structure

N Nakatani, H Sakuta, M Hayashi, S Tanaka… - …, 2018 - Wiley Online Library
The effect of binary hydrophilic polymers on a pair of representative bio‐macromolecules in
a living cell has been examined. The results showed that these bio‐macromolecules …