Abstract Mutations in codon 12, 13, or 61 of one of the three ras genes, H-ras, K-ras, and N-
ras, convert these genes into active oncogenes. Rapid assays for the detection of these …

The search for genetic damage in neoplastic cells now occupies a central place in cancer
research. Diverse examples of such damage are in hand, and they in turn hint at …

Using in vitro gene amplification by the polymerase chain reaction (PCR) and mutation
detection by the RNAase A mismatch cleavage method, we have examined cK-ras genes in …

Abstract The Department of Medical Biochemistry and t Department of Organic Chemistry,
State University of Leiden, Sylvius Laboratories, The Netherlands* The Oncology Center …

Mutations of the p53 gene occur commonly in colorectal carcinomas and the wild-type p53
allele is often concomitantly deleted. These findings suggest that the wild-type gene may act …

We report a rapid and sensitive method for the detection of base changes in given
sequences of genomic DNA. This technique is based on the facts that specific regions of …

An assay for the presence of given DNA sequences has been developed, based on the
ability of two oligonucleotides to anneal immediately adjacent to each other on a …

Allele loss is a hallmark of chromosome regions harboring recessive oncogenes. Lung
cancer frequently demonstrates loss of heterozygosity on 17p. Recent evidence suggests …

The epidermal growth factor receptor (EGFR) gene is amplified in 40% of malignant gliomas,
and the amplified genes are frequently rearranged. We have characterized the genetic …

We examined somatic mutations of the adenomatous polyposis coil (APC) gene in 63
colorectal tumors (16 adenomas and 47 carcinomas) developed in familial adenomatous …