Best practices for analysing microbiomes

R Knight, A Vrbanac, BC Taylor, A Aksenov… - Nature Reviews …, 2018 - nature.com
Complex microbial communities shape the dynamics of various environments, ranging from
the mammalian gastrointestinal tract to the soil. Advances in DNA sequencing technologies …

A review of methods and databases for metagenomic classification and assembly

FP Breitwieser, J Lu, SL Salzberg - Briefings in bioinformatics, 2019 - academic.oup.com
Microbiome research has grown rapidly over the past decade, with a proliferation of new
methods that seek to make sense of large, complex data sets. Here, we survey two of the …

IMG/M v. 5.0: an integrated data management and comparative analysis system for microbial genomes and microbiomes

IMA Chen, K Chu, K Palaniappan, M Pillay… - Nucleic acids …, 2019 - academic.oup.com
Abstract The Integrated Microbial Genomes & Microbiomes system v. 5.0 (IMG/M: https://img.
jgi. doe. gov/m/) contains annotated datasets categorized into: archaea, bacteria, eukarya …

MetaWRAP—a flexible pipeline for genome-resolved metagenomic data analysis

GV Uritskiy, J DiRuggiero, J Taylor - Microbiome, 2018 - Springer
Background The study of microbiomes using whole-metagenome shotgun sequencing
enables the analysis of uncultivated microbial populations that may have important roles in …

Rhizosphere microbiome structure alters to enable wilt resistance in tomato

MJ Kwak, HG Kong, K Choi, SK Kwon, JY Song… - Nature …, 2018 - nature.com
Tomato variety Hawaii 7996 is resistant to the soil-borne pathogen Ralstonia solanacearum,
whereas the Moneymaker variety is susceptible to the pathogen. To evaluate whether plant …

Integrating cultivation and metagenomics for a multi-kingdom view of skin microbiome diversity and functions

S Saheb Kashaf, DM Proctor, C Deming, P Saary… - Nature …, 2022 - nature.com
Human skin functions as a physical barrier to foreign pathogen invasion and houses
numerous commensals. Shifts in the human skin microbiome have been associated with …

A deep siamese neural network improves metagenome-assembled genomes in microbiome datasets across different environments

S Pan, C Zhu, XM Zhao, LP Coelho - Nature communications, 2022 - nature.com
Metagenomic binning is the step in building metagenome-assembled genomes (MAGs)
when sequences predicted to originate from the same genome are automatically grouped …

Assembly of 913 microbial genomes from metagenomic sequencing of the cow rumen

RD Stewart, MD Auffret, A Warr, AH Wiser… - Nature …, 2018 - nature.com
The cow rumen is adapted for the breakdown of plant material into energy and nutrients, a
task largely performed by enzymes encoded by the rumen microbiome. Here we present …

Critical assessment of metagenome interpretation—a benchmark of metagenomics software

A Sczyrba, P Hofmann, P Belmann, D Koslicki… - Nature …, 2017 - nature.com
Methods for assembly, taxonomic profiling and binning are key to interpreting metagenome
data, but a lack of consensus about benchmarking complicates performance assessment …

The lichens' microbiota, still a mystery?

M Grimm, M Grube, U Schiefelbein, D Zühlke… - Frontiers in …, 2021 - frontiersin.org
Lichens represent self-supporting symbioses, which occur in a wide range of terrestrial
habitats and which contribute significantly to mineral cycling and energy flow at a global …