Fluorescence lifetime measurements and biological imaging

MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

[HTML][HTML] Recent advances in using liposomes for delivery of nucleic acid-based therapeutics

H Nsairat, W Alshaer, F Odeh, E Esawi, D Khater… - OpenNano, 2023 - Elsevier
Nucleic acid therapeutics are rapidly expanding because of recent advancements in
production and purification. This class of therapeutics may change the field of disease …

Single-vesicle assays using liposomes and cell-derived vesicles: from modeling complex membrane processes to synthetic biology and biomedical applications

H Pick, AC Alves, H Vogel - Chemical reviews, 2018 - ACS Publications
The plasma membrane is of central importance for defining the closed volume of cells in
contradistinction to the extracellular environment. The plasma membrane not only serves as …

[HTML][HTML] Cholesterol-induced fluid membrane domains: a compendium of lipid-raft ternary phase diagrams

D Marsh - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2009 - Elsevier
The biophysical underpinning of the lipid-raft concept in cellular membranes is the liquid-
ordered phase that is induced by moderately high concentrations of cholesterol. Although …

Ultrathin shell double emulsion templated giant unilamellar lipid vesicles with controlled microdomain formation

LR Arriaga, SS Datta, SH Kim, E Amstad, TE Kodger… - small, 2014 - Wiley Online Library
A microfluidic approach is reported for the high‐throughput, continuous production of giant
unilamellar vesicles (GUVs) using water‐in‐oil‐in‐water double emulsion drops as …

[HTML][HTML] Lipid lateral diffusion and membrane heterogeneity

G Lindblom, G Orädd - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2009 - Elsevier
The pulsed field gradient (pfg)-NMR method for measurements of translational diffusion of
molecules in macroscopically aligned lipid bilayers is described. This technique is proposed …

Using confocal laser scanning microscopy to probe the milk fat globule membrane and associated proteins

S Gallier, D Gragson, R Jiménez-Flores… - Journal of Agricultural …, 2010 - ACS Publications
The bovine milk fat globule membrane (MFGM) is an important, biologically relevant
membrane due to its functional and health properties. Its composition has been thoroughly …

Phase diagrams of lipid mixtures relevant to the study of membrane rafts

FM Goñi, A Alonso, LA Bagatolli, RE Brown… - … et Biophysica Acta (BBA …, 2008 - Elsevier
The present paper reviews the phase properties of phosphatidylcholine–sphingomyelin–
cholesterol mixtures, that are often used as models for membrane “raft” microdomains. The …

Phase equilibria in DOPC/DPPC-d62/cholesterol mixtures

JH Davis, JJ Clair, J Juhasz - Biophysical journal, 2009 - cell.com
There is broad interest in the question of fluid-fluid phase coexistence in membranes, in
particular, whether evidence for liquid-disordered (ld)-liquid-ordered (lo) two-phase regions …

Non-invasive imaging of skin physiology and percutaneous penetration using fluorescence spectral and lifetime imaging with multiphoton and confocal microscopy

MS Roberts, Y Dancik, TW Prow, CA Thorling… - European Journal of …, 2011 - Elsevier
New multiphoton and confocal microscope technologies and fluorescence lifetime imaging
techniques are now being used to non-invasively image, in space (three dimensions), in …