Fluorescence lifetime measurements and biological imaging
MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …
[HTML][HTML] Recent advances in using liposomes for delivery of nucleic acid-based therapeutics
Nucleic acid therapeutics are rapidly expanding because of recent advancements in
production and purification. This class of therapeutics may change the field of disease …
production and purification. This class of therapeutics may change the field of disease …
Single-vesicle assays using liposomes and cell-derived vesicles: from modeling complex membrane processes to synthetic biology and biomedical applications
The plasma membrane is of central importance for defining the closed volume of cells in
contradistinction to the extracellular environment. The plasma membrane not only serves as …
contradistinction to the extracellular environment. The plasma membrane not only serves as …
[HTML][HTML] Cholesterol-induced fluid membrane domains: a compendium of lipid-raft ternary phase diagrams
D Marsh - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2009 - Elsevier
The biophysical underpinning of the lipid-raft concept in cellular membranes is the liquid-
ordered phase that is induced by moderately high concentrations of cholesterol. Although …
ordered phase that is induced by moderately high concentrations of cholesterol. Although …
Ultrathin shell double emulsion templated giant unilamellar lipid vesicles with controlled microdomain formation
A microfluidic approach is reported for the high‐throughput, continuous production of giant
unilamellar vesicles (GUVs) using water‐in‐oil‐in‐water double emulsion drops as …
unilamellar vesicles (GUVs) using water‐in‐oil‐in‐water double emulsion drops as …
[HTML][HTML] Lipid lateral diffusion and membrane heterogeneity
G Lindblom, G Orädd - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2009 - Elsevier
The pulsed field gradient (pfg)-NMR method for measurements of translational diffusion of
molecules in macroscopically aligned lipid bilayers is described. This technique is proposed …
molecules in macroscopically aligned lipid bilayers is described. This technique is proposed …
Using confocal laser scanning microscopy to probe the milk fat globule membrane and associated proteins
S Gallier, D Gragson, R Jiménez-Flores… - Journal of Agricultural …, 2010 - ACS Publications
The bovine milk fat globule membrane (MFGM) is an important, biologically relevant
membrane due to its functional and health properties. Its composition has been thoroughly …
membrane due to its functional and health properties. Its composition has been thoroughly …
Phase diagrams of lipid mixtures relevant to the study of membrane rafts
The present paper reviews the phase properties of phosphatidylcholine–sphingomyelin–
cholesterol mixtures, that are often used as models for membrane “raft” microdomains. The …
cholesterol mixtures, that are often used as models for membrane “raft” microdomains. The …
Phase equilibria in DOPC/DPPC-d62/cholesterol mixtures
There is broad interest in the question of fluid-fluid phase coexistence in membranes, in
particular, whether evidence for liquid-disordered (ld)-liquid-ordered (lo) two-phase regions …
particular, whether evidence for liquid-disordered (ld)-liquid-ordered (lo) two-phase regions …
Non-invasive imaging of skin physiology and percutaneous penetration using fluorescence spectral and lifetime imaging with multiphoton and confocal microscopy
New multiphoton and confocal microscope technologies and fluorescence lifetime imaging
techniques are now being used to non-invasively image, in space (three dimensions), in …
techniques are now being used to non-invasively image, in space (three dimensions), in …