Growth-mediated feedback between synthetic gene circuits and host organisms leads to
diverse emerged behaviors, including growth bistability and enhanced ultrasensitivity …

Failure of modularity remains a significant challenge for assembling synthetic gene circuits
with tested modules as they often do not function as expected. Competition over shared …

Broad-host-range synthetic biology is an emerging frontier that aims to expand our current
engineerable domain of microbial hosts for biodesign applications. As more novel species …

Multicellular systems feature a stunning array of complex emergent behaviors that are
compelling engineering targets. These behaviors often arise from cells taking on different …

Growth feedback, the inherent coupling between the synthetic gene circuit and the host cell
growth, could significantly change the circuit behaviors. Previously, a diverse array of …

Bistable switches that produce all-or-none responses have been found to regulate a number
of natural cellular decision making processes, and subsequently synthetic switches were …

Multistability and natural biological variability can result in significant heterogeneity within a
cell population, leading to challenges in understanding and modulating cell behavior …

Cells often encounter various external and internal signals in a non-sustained pulsatile
manner with varying amplitude, duration and residual value. However, the effect of signal …

A notable challenge when assembling synthetic gene circuits is that modularity often fails to
function as intended. A crucial underlying reason for this modularity failure is the existence …

The mutual inhibition between synthetic gene circuits and cell growth produces growth
feedback in the host-circuit system. Previous studies have demonstrated that the growth …